FGF7 Protein (AA 32-194) (His tag)

Produktnummer ABIN2018184

Produktdetails

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 32-194

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses FGF7 Protein ist gelabelt mit His tag.

Produktmerkmale: ED50 < 10 ng/mL, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 1.0x 10^5 units/mg.
AA 32 -194, expressed with N-terminal His-Tag.

Reinheit: > 95 % by SDS-PAGE and HPLC analyses.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant human Keratinocyte Growth Factor (rhKGF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhKGF should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Andere Bezeichnung: Keratinocyte Growth Factor (KGF/FGF-7) (FGF7 Produkte)

Synonyme: HBGF-7 Protein, KGF Protein, Kgf Protein, kgf Protein, FGF-7 Protein, fgf7 Protein, fibroblast growth factor 7 Protein, fibroblast growth factor 7 L homeolog Protein, FGF7 Protein, Fgf7 Protein, fgf7 Protein, fgf7.L Protein

Hintergrund: Keratinocyte Growth Factor (KGF) is a highly specific epithelial mitogen produced by fibroblasts and mesenchymal stem cells. KGF belongs to the heparin binding Fibroblast Growth Factor (FGF) family, and is known as FGF-7. However, in contrast to the FGF-1, which binds to all known FGF receptors with high affinity, KGF only binds to a splice variant of an FGF receptor, FGFR2-IIIb. FGFR2-IIIb is produced by most of the epithelial cells, indicating that KGF plays roles as a paracrine mediator. KGF induces the differen-tiation and proliferation of various epithelial cells, including keratinocytes in the epidermis, hair follicles and sebaceous glands, and is responsible for the wound repairs of various tissues, including lung, bladder, and kidney. Recombinant human Keratinocyte Growth Factor (rhKGF) with N-terminal His-tag produced in E. coli is a single non-glycosylated polypeptide chain containing 181 amino acids. A fully biologically active molecule, rhKGF has a molecular mass of 21.2 kDa analyzed by reducing SDS-PAGE.
Synonyms: Keratinocyte Growth Factor, Fibroblast Growth Factor-7, HBGF-7

Molekulargewicht: 21.2 kDa, observed by reducing SDS-PAGE.

UniProt: P21781

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung