FGF4 Protein (His tag)

Produktnummer ABIN1589631

Produktdetails

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses FGF4 Protein ist gelabelt mit His tag.

Sequenz: MGHHHHHHHH HHSSGHIEGR HMAPNGTRHA ELGHGWDGLV ARSLARLPVA AQPPQAAVRS GAGDYLLGLK RLRRLYCNVG IGFHLQVLPD GRIGGVHADT RDSLLELSPV QRGVVSIFGV ASRFFVAMSS RGKLFGVPFF TDECKFKEIL LPNNYNAYEA YAYPGMFMAL SKNGRTKKGN RVSPTMKVTH FLPRL

Produktmerkmale: Length (AA): 195
Chromosomal location: 7 F5, 7 724.4 cM
The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).

Reinheit: > 95 % by SDS-PAGE. Visualized by silver stain

Anwendungsinformationen

Kommentare:

Cytokines & Growth Factors

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution in water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or -20 °C for future use.

Buffer: 50 mM MES, 100 mM NaCl

Handhabung: Centrifuge vial prior to opening. Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20 °C. Reconstituted FGF-4 should be stored in working aliquots at -20 °C.

Antigendetails

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Andere Bezeichnung: FGF-4 (FGF4 Produkte)

Synonyme: HBGF-4 Protein, HST Protein, HST-1 Protein, HSTF1 Protein, K-FGF Protein, KFGF Protein, Fgf-4 Protein, Fgfk Protein, Hst1 Protein, Hstf-1 Protein, KS3 Protein, hst Protein, hst-1 Protein, kFGF Protein, Hst Protein, fibroblast growth factor 4 Protein, FGF4 Protein, Fgf4 Protein

Hintergrund: FGF4 (fibroblast growth factor4), also known as FGF-K or K-FGF (Kaposi’s sarcoma-associated FGF), is a 25 kDa secreted, heparin-binding member of the FGF family. The mouse FGF4 cDNA encodes 202 amino acids (aa) with a 29 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus. Mature mouse FGF 4 shares 87%, 90 %, 87% and 85 % aa identity with human, rat, canine and bovine FGF4, respectively. Human FGF4 has been shown to exhibit cross species activity. Expression of FGF4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development. Its expression in the trophoblast inner cell mass promotes expression of FGF R2, and is required for maintenance of the trophectoderm and primitive endoderm. Later in development, FGF4 works together with FGF8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of vertebrate limbs. FGF4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro. A C-terminally truncated 15 kDa isoform that opposes full length FGF4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential. It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease.
Synonyms: Fgf4, KS3, hst, Fgfk, Hst1, kFGF, Fgf-4, hst-1, Hstf-1

Molekulargewicht: 21.6 kDa

NCBI Accession: NM_010202, NP_034332

UniProt: P11403

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance