CCL2 Protein
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- Target Alle CCL2 Proteine anzeigen
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- Protein-Typ
- Recombinant
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Applikation
- ELISA, Functional Studies (Func)
- Marke
- BD Pharmingen™
- Spezifität
- Bioactivity: This material has been reported to have bioactivity when measured by Ca 2+ Flux Assay, using Human THP-1cells. The EC50 is commonly in the range of 20-350 ng/ml. Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
- Produktmerkmale
- Recombinant human MCP-1 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution (pH 7.2) and containing 2 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The human MCP-1 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.
- Aufreinigung
- Recombinant human MCP-1 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution and bovine serum albumin, with no preservatives. Recombinant human MCP-1 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.
- Reinheit
- ≥ 95 %
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- The endotoxin level is ≤ 0.1 ng per µg of human MCP-1, as measured in a chromogenic LAL assay.
- Top Product
- Discover our top product CCL2 Protein
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- Applikationshinweise
- ELISA Standard: The human MCP-1 (Cat. No. 554620) is useful as a quantitative standard for measuring human MCP-1 protein levels in a sandwich ELISA with the purified 10F7 antibody (Cat. No. 555055) as a capture antibody and the biotinylated 5D3-F7 (Cat. No. 554664) as the detection antibody. To obtain linear standard curves, doubling dilutions of this human MCP-1 standard from 2000-4 pg/ml should be included in each ELISA plate.
- Kommentare
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BD Pharmingen™ Recombinant Human MCP-1
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 200 μg/mL
- Buffer
- Frozen aqueous buffered solution containing BSA.
- Konservierungsmittel
- Azide free
- Handhabung
- Human monocyte chemoattractant protein-1 (MCP-1), also known as MCAF (monocyte chemotactic and activating factor), is a member of CC chemokine family. MCP-1 is produced by a variety of stimulated cell types including monocytes, lymphocytes, endothelial cells and fibroblasts. MCP-1 is a potent chemoattractant for monocytes and it also activates lymphocytes, basophils and NK cells.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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- Target
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- Andere Bezeichnung
- MCP-1 (CCL2 Produkte)
- Synonyme
- GDCF-2 Protein, HC11 Protein, HSMCR30 Protein, MCAF Protein, MCP-1 Protein, MCP1 Protein, SCYA2 Protein, SMC-CF Protein, AI323594 Protein, JE Protein, Scya2 Protein, Sigje Protein, MCP-1A Protein, MCP1A Protein, C-C motif chemokine ligand 2 Protein, chemokine (C-C motif) ligand 2 Protein, C-C motif chemokine 2 Protein, CCL2 Protein, Ccl2 Protein, LOC101120093 Protein
- Hintergrund
- Human monocyte chemoattractant protein-1 (MCP-1), also known as MCAF (monocyte chemotactic and activating factor), is a member of CC chemokine family. MCP-1 is produced by a variety of stimulated cell types including monocytes, lymphocytes, endothelial cells and fibroblasts. MCP-1 is a potent chemoattractant for monocytes and it also activates lymphocytes, basophils and NK cells.
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylierungen bei SARS-CoV-2 Infektion
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