CPSF4 Protein (His tag)

Produktnummer ABIN1098798

Produktdetails

Target: CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses CPSF4 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Reinheit: > 85 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: cleavage and polyadenylation specific factor 4, 30kDa, CPSF30, NAR, NEB1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 M UREA, 10% glycerol

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))

Andere Bezeichnung: CPSF4 (CPSF4 Produkte)

Synonyme: cpsf30 Protein, nar Protein, neb1 Protein, 30kDa Protein, C79664 Protein, CPSF30 Protein, NAR Protein, NEB1 Protein, cleavage and polyadenylation specific factor 4 Protein, cleavage and polyadenylation specific factor 4, 30kDa Protein, cleavage and polyadenylation specific factor 4 S homeolog Protein, CPSF4 Protein, Cpsf4 Protein, LOC733099 Protein, LOC100360200 Protein, cpsf4.S Protein, cpsf4 Protein

Hintergrund: Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of CPSF4 an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Recombinant human CPSF4 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molekulargewicht: 29.9 kDa (267aa)

NCBI Accession: NP_001075028