TNF alpha Protein (AA 77-233)

Produktnummer ABIN1097668

Dieses Recombinant TNF alpha-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für TNF alpha Protein (AA 77-233) (ABIN1097668)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 77-233

Verwendungszweck: Recombinant Human TNF alpha

Sequenz: MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

Produktmerkmale: Recombinant Human Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, pH 7.0.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-5 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 3 months

Antigendetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor Necrosis Factor alpha/tnf-alpha

Hintergrund: Alternative Names: Tumor Necrosis Factor, Cachectin, TNF-Alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFA, TNFSF2Recombinant Tumor Necrosis Factor α/TNF-α is a non-glycosylated cytokine produced from E. coli using rDNA technology. The protein consists of three identical polypeptide chains of 158 AA combined to form a compact bell-shaped homotrimer. The individual subunits have a relative molecular mass of 17,484 Daltons each. TNF-α is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
TNFα is a homotrimer with a subunit molecular mass of 17 kDa and plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune diseases and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease.

Molekulargewicht: 17.3 kDa

UniProt: P01375

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome