TNF alpha Protein (AA 89-235)

Produktnummer ABIN7320677

Dieses Recombinant TNF alpha-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für TNF alpha Protein (AA 89-235) (ABIN7320677)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Cell Culture (CC)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 89-235

Verwendungszweck: Recombinant Mouse TNF-alpha/TNFA Protein

Sequenz: Asp89-Leu235

Produktmerkmale: Recombinant Mouse Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Asp89-Leu235 is expressed.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 0.01 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 2-8 pg/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF-alpha/TNFA

Hintergrund: Cachectin,DIF,Membrane Form,TNF-Alpha,TNF-a,TNF-alpha,TNFSF2,TNFalpha,Tnf,Tnfa,Tnfsf1a,Tnfsf2,Tumor Necrosis Factor,Tumor Necrosis Factor Ligand Superfamily Member 2,Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.

Molekulargewicht:

Calculated MW: 16.4 kDa

Observed MW: 14 kDa

Gen-ID: 21926

UniProt: P06804

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome