recombinant ADAM 10 comprises a 459 amino acid fragment (214-672) corresponding to the ADAM 10 extracellular domain minus the signal peptide and prosequence and is expressed in E. coli with an aminoterminal hexahistidine tag.
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Hintergrund
ADAM 10 is a member of the ADAM family of membraneanchored cell surface proteins, which contain both a disintegrin and a metalloprotease domain. ADAM 10 is expressed in a wide variety of tissues and resides on both the cell surface and within the cytoplasm. ADAM10 has been implicated in proteolytic release of a number of cell surface proteins in particular TNF alpha,AD10, KUZ, EC 3.4.24.81, A desintegrin and metalloprotease domain 10, mammalian disintegrinmetalloprotease, Kuzbanian protein homolog, CDw156c antigen