anti-Human PDPK1 Antikörper für Immunofluorescence

Recommended PDPK1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antikörper
  • pdpk1
  • MGC82080
  • PDK1
  • PDPK2
  • PRO0461
  • Pdk1
  • si:zfos-979f1.3
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • 3'-phosphoinositide-dependent protein kinase 1
  • 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1
  • ATPDK1
  • AtPDK1
  • T32M21.110
  • T32M21_110
  • 3-phosphoinositide dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase 1 L homeolog
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide-dependent protein kinase 1
  • pyruvate dehydrogenase kinase, isozyme 1
  • pyruvate dehydrogenase kinase 1
  • Phosphoinositide-dependent kinase 1
  • 3'-phosphoinositide-dependent protein kinase 1
  • PDPK1
  • pdpk1.L
  • pdpk1
  • Pdpk1
  • LOC100380750
  • pdk1
  • PDK1
  • Pdk1
  • pdk-1
Epitop
C-Term, AA 350-436
59
35
27
27
26
26
15
15
15
15
13
12
7
7
6
6
6
5
5
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
452
222
221
19
13
12
11
11
6
5
4
3
2
2
2
1
1
1
1
1
Wirt
Kaninchen
372
73
16
Klonalität
Polyklonal
Konjugat
Dieser PDPK1 Antikörper ist unkonjugiert
17
17
14
11
11
7
7
7
7
7
7
7
7
7
6
5
5
4
3
3
3
3
3
2
2
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
308
163
104
91
66
65
40
32
21
10
6
5
3
2
1
1
Hersteller
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Bilder

Produktnummer ABIN250776
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
11.708044 ABIN1874088 IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
11.708044 ABIN1870500 IF IHC WB Rabbit IgG pSer241 Anmelden zum Anzeigen Polyclonal 0
11.708044 ABIN450008 ELISA FACS ICC IF WB Mouse IgG1 kappa AA 29-436 Anmelden zum Anzeigen 2D6 0
11.708044 ABIN4344570 ICC IF IHC IHC (p) WB Rabbit pSer241 Anmelden zum Anzeigen Polyclonal 0
11.708044 ABIN4344573 ICC IF IP WB Rabbit IgG Center Anmelden zum Anzeigen Polyclonal 0
11.708044 ABIN1108608 EIA FACS IF IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 4A11 3
11.708044 ABIN5078734 ICC IF Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
11.708044 ABIN4344574 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 4A11 0
11.708044 ABIN4344641 ICC IF IHC IHC (p) Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
11.708044 ABIN5078729 ICC IF Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
11.708044 ABIN5545315 IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
11.708044 ABIN3020427 IF IHC WB Rabbit pSer241 Anmelden zum Anzeigen Polyclonal 0
1 ABIN197100 IF IHC (p) WB Rabbit Ser241 Anmelden zum Anzeigen Polyclonal 5
1 ABIN968352 IF WB Mouse IgG1 AA 439-555 Anmelden zum Anzeigen 5-PDK1 5
1 ABIN968351 IF WB Mouse IgG1 AA 439-555 Anmelden zum Anzeigen 5-PDK1 5
1 ABIN196616 IF IHC (p) WB Rabbit pSer241 Anmelden zum Anzeigen Polyclonal 4
1 ABIN361882 IF IHC WB Rabbit IgG pSer241 Anmelden zum Anzeigen Polyclonal 6
1 ABIN362492 IF IHC WB Rabbit IgG AA 239-243 Anmelden zum Anzeigen Polyclonal 6
1 ABIN5689224 IF IHC WB Rabbit AA 239-243 Anmelden zum Anzeigen Polyclonal 0
1 ABIN5689780 IF IHC WB Rabbit pSer241 Anmelden zum Anzeigen Polyclonal 0

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antikörper
Epitop C-Term, AA 350-436
(59), (35), (27), (27), (26), (26), (15), (15), (15), (15), (13), (12), (7), (7), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(452), (222), (221), (19), (13), (12), (11), (11), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Wirt Kaninchen
(372), (73), (16)
Klonalität
Polyklonal
Konjugat Dieser PDPK1 Antikörper ist unkonjugiert
(17), (17), (14), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(308), (163), (104), (91), (66), (65), (40), (32), (21), (10), (6), (5), (3), (2), (1), (1)
Pubmed 3 Publikationen vorhanden
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Produktdetails anti-PDPK1 Antikörper

Target Details PDPK1 Anwendungsinformationen Handhabung Referenzen für anti-PDPK1 Antikörper (ABIN250776) Bilder
Produktmerkmale PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1) plays a role in glucose and metabolism of fatty acid regulation via phosphorylation of the pyruvate dehydrogenase subunits. PDK1 also inhibits the activity of pyruvate dehydrogenase and helps regulate metabolite change. PDK1 cellular response to hypoxia is important for cell proliferation under hypoxic conditions. PDK1 also protects against apoptosis in response to oxidative stress and conditions of low oxygen.
Reinigung Immunogen affinity purified
Immunogen A synthetic peptide made to a C-terminal portion of the human PDK1 protein sequence (between residues 350-436). [Swiss-Prot Q15118]

Target Details PDPK1

Produktdetails anti-PDPK1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-PDPK1 Antikörper (ABIN250776) Bilder zurück nach oben
Antigen
Andere Bezeichnung PDK-1 (PDPK1 Antibody Abstract)
Hintergrund Gene Symbol: PDK1
Molekulargewicht Theoretical MW: 49 kDa
Gen-ID 5163
UniProt Q15118
Pathways PI3K-Akt Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Anwendungsinformationen

Produktdetails anti-PDPK1 Antikörper Target Details PDPK1 Handhabung Referenzen für anti-PDPK1 Antikörper (ABIN250776) Bilder zurück nach oben
Applikationshinweise Western Blot 2 μg/mL, Immunocytochemistry/Immunofluorescence 1:1000-1:2000This PDK1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot. By Western blot a band is seen at ~49 kDa, representing PDK1 protein. There are also two higher and one lower non-specific molecular weight bands. In ICC/IF, mitochondiral staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for PDK1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour at room temperature (RT).
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-PDK1 primary antibody in blocking buffer and incubate 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-PDPK1 Antikörper Target Details PDPK1 Anwendungsinformationen Referenzen für anti-PDPK1 Antikörper (ABIN250776) Bilder zurück nach oben
Format Liquid
Konzentration 1.42 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung -80 °C,-20 °C
Informationen zur Lagerung Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Referenzen für anti-PDPK1 Antikörper (ABIN250776)

Produktdetails anti-PDPK1 Antikörper Target Details PDPK1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Tsoi, Li, Chen, Lau, Tsui, Chan: "The SARS-coronavirus membrane protein induces apoptosis via interfering with PDK1-PKB/Akt signalling." in: The Biochemical journal, Vol. 464, Issue 3, pp. 439-47, 2014 (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Luo, Chang, Zhong, Pandey, Semenza: "Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 49, pp. E3367-76, 2012

Luo, Hu, Chang, Zhong, Knabel, OMeally, Cole, Pandey, Semenza: "Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1." in: Cell, Vol. 145, Issue 5, pp. 732-44, 2011

Bilder

Produktdetails anti-PDPK1 Antikörper Target Details PDPK1 Anwendungsinformationen Handhabung Referenzen für anti-PDPK1 Antikörper (ABIN250776) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 350-436), (C-Term) antibody (ABIN250776) Detection of PDK1 in human heart lysate using ABIN250776 at 2 ug/ml.
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 350-436), (C-Term) antibody (ABIN250776) Western Blot: PDK-1 Antibody [ABIN250776] - Analysis of HeLa lysates using ABIN250776...
Immunofluorescence (IF) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 350-436), (C-Term) antibody (ABIN250776) Immunocytochemistry/Immunofluorescence: PDK-1 Antibody [ABIN250776] - Antibody was te...