anti-Ratte (Rattus) AKT Antikörper für Western Blotting

Recommended AKT Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antikörper
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitop
AA 445-480
230
119
113
58
58
51
36
33
33
25
23
22
21
19
18
18
14
14
12
12
11
11
11
11
10
10
10
9
9
8
7
7
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
1328
752
591
64
57
56
54
37
31
28
7
6
6
5
4
3
3
3
1
1
1
1
1
Wirt
Kaninchen
1024
389
11
4
2
1
Klonalität
Polyklonal
Konjugat
Dieser AKT Antikörper ist unkonjugiert
56
43
43
40
33
26
15
15
10
10
8
6
6
6
6
6
6
6
6
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1215
617
595
267
203
201
138
90
64
38
32
25
12
11
10
4
4
3
3
2
2
1
1
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN725195
$ 318.45
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.199711 ABIN4900619 CyTOF FACS IHC SimWes WB Rabbit IgG pSer473 Anmelden zum Anzeigen Polyclonal 16
3.199711 ABIN223011 IP IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 2
3.199711 ABIN94783 FM IHC (fp) IP ELISA WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 2
3.199711 ABIN3043108 WB Mouse IgG1 AA 461-477 Anmelden zum Anzeigen PKB-175 2
3.199711 ABIN1043753 IP IHC ELISA WB Biotin Mouse IgG1 kappa pThr308, Internal Region Anmelden zum Anzeigen 18F3-H11 4
3.199711 ABIN1043752 IP IHC ELISA WB Biotin Mouse IgG1 kappa pSer473, Internal Region Anmelden zum Anzeigen 17F6-B11 4
3.199711 ABIN2792187 WB Rabbit N-Term Anmelden zum Anzeigen Polyclonal 1
3.199711 ABIN2792186 WB Rabbit N-Term Anmelden zum Anzeigen Polyclonal 1
3.199711 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Anmelden zum Anzeigen Polyclonal 3
3.199711 ABIN223018 IP WB Rabbit IgG pSer473 Anmelden zum Anzeigen Polyclonal 6
3.199711 ABIN1043756 FM FRET STED ELISA WB Atto 594 Mouse IgG1 kappa pSer473, Internal Region Anmelden zum Anzeigen 17F6-B11 4
3.199711 ABIN2707319 IHC WB Rabbit N-Term Anmelden zum Anzeigen Polyclonal 0
3.199711 ABIN2957853 WB Rabbit pTyr326 Anmelden zum Anzeigen Polyclonal 0
3.199711 ABIN2705427 IHC WB Rabbit Center Anmelden zum Anzeigen Polyclonal 0
3.199711 ABIN2705425 IC IF IP WB Rabbit pSer308 Anmelden zum Anzeigen Polyclonal 0
3.199711 ABIN2705430 IC IF IHC WB Rabbit Center Anmelden zum Anzeigen Polyclonal 0
3.199711 ABIN2705426 IC IF IHC WB Rabbit pTyr315 Anmelden zum Anzeigen Polyclonal 0
3.199711 ABIN2705428 IP IHC WB Rabbit Center Anmelden zum Anzeigen Polyclonal 0
3.199711 ABIN2705429 IHC WB Rabbit Center Anmelden zum Anzeigen Polyclonal 0
3.199711 ABIN2704463 IP IHC WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antikörper
Epitop AA 445-480
(230), (119), (113), (58), (58), (51), (36), (33), (33), (25), (23), (22), (21), (19), (18), (18), (14), (14), (12), (12), (11), (11), (11), (11), (10), (10), (10), (9), (9), (8), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(1328), (752), (591), (64), (57), (56), (54), (37), (31), (28), (7), (6), (6), (5), (4), (3), (3), (3), (1), (1), (1), (1), (1)
Wirt Kaninchen
(1024), (389), (11), (4), (2), (1)
Klonalität
Polyklonal
Konjugat Dieser AKT Antikörper ist unkonjugiert
(56), (43), (43), (40), (33), (26), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1215), (617), (595), (267), (203), (201), (138), (90), (64), (38), (32), (25), (12), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-AKT Antikörper

Target Details AKT Anwendungsinformationen Handhabung Referenzen für anti-AKT Antikörper (ABIN725195) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human AKT1
Isotyp IgG
Plasmids, Primers & others

Target Details AKT

Produktdetails anti-AKT Antikörper Anwendungsinformationen Handhabung Referenzen für anti-AKT Antikörper (ABIN725195) Bilder zurück nach oben
Antigen
Andere Bezeichnung AKT1 (AKT1 Antibody Abstract)
Hintergrund

AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility, prohibitin (PHB), playing an important role in cell metabolism and proliferation, and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Subcellular location: Cytoplasm, Nucleus

Synonyms: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, PKBG

Gen-ID 207
UniProt P31749
Pathways PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Anwendungsinformationen

Produktdetails anti-AKT Antikörper Target Details AKT Handhabung Referenzen für anti-AKT Antikörper (ABIN725195) Bilder zurück nach oben
Applikations-hinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-AKT Antikörper Target Details AKT Anwendungsinformationen Referenzen für anti-AKT Antikörper (ABIN725195) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Referenzen für anti-AKT Antikörper (ABIN725195)

Produktdetails anti-AKT Antikörper Target Details AKT Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, 2018 Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Mouse (Murine)). Weitere Details: Sample: testicular tissue

Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." in: Scientific reports, Vol. 6, pp. 37884, 2016 Von den Autoren verwendete Methode: Western Blotting (WB), Western Blotting (WB) (Probematerial (Species): Pig (Porcine)).

Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." in: Bioscience reports, Vol. 34, Issue 5, 2014 Von den Autoren verwendete Methode: Western Blotting (WB)

Xu, Huang, Xie, Lin, Guo, Zhuang, Lin, Zhou, Li, Huang, Wei, Man, Zhang: "The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients." in: BMC cancer, Vol. 13, pp. 247, 2013 Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Human). Weitere Details: Sample: glioma tissue

Bilder

Produktdetails anti-AKT Antikörper Target Details AKT Anwendungsinformationen Handhabung Referenzen für anti-AKT Antikörper (ABIN725195) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Mouse placenta lysate with Anti-AKT1/3 Polyclonal Antibody Unconjugated at 1:5000 fo...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Lane 1: Mouse Placenta lysates;Lane 2: Mouse Lung lysates probed with AKT1/3 Polyclon...
'Independent Validation' Siegel
Chargennummer 131113
Validierte Anwendung Flow Cytometry
Positivkontrolle MDA-MB-468 cells - lower expression
Bewertung A strong specific signal is observed in both of the positive control cells stained with anti-AKT1 plus secondary antibody compared with isotype, secondary only and unstained cells.
Primärantikörper
  • Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
  • Catalog number: ABIN725195
  • Supplier: Anmelden zum Anzeigen
  • Supplier catalog number: Anmelden zum Anzeigen
  • Lot number: 131113
  • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: MCF-7 cells
  • Low positive control: MDA-MB-468 cells
  • Isotype control: Both cell lines treated with rabbit IgG instead of the primary antibody to confirm that primary antibody binding is specific.
  • Secondary only control: Both cell lines treated with Goat anti-rabbit IgG-FITC to confirm no background signal produced from secondary antibody alone
Protokoll
  • Control cells were cultured in DMEM + 10% FBS. - Control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
  • Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
  • An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37 °C.
  • Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
  • Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA.
  • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
  • Cells were incubated for 30 min on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
  • Goat anti-rabbit IgG-FITC secondary antibody (Jackson Immunoresearch) was added at a 1:200 dilution. The cells were incubated for 30 min in the dark on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Cells were analyzed on a FACSAria III (BD Biosciences) using a blue laser (488 nm excitation / 525 nm emission).
Anmerkungen Nothing to note.
Validierungsbilder
Flow Cytometry validation image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Figure 1: Positive control (high expression) MCF7 cells. The red histogram is unstain...
Flow Cytometry validation image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Figure 2: Positive control (lower expression) MDA-MB-468 cells. The red histogram is ...