anti-Maus AKT Antikörper für ELISA

Recommended AKT Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antikörper
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitop
pSer473, Internal Region
235
117
113
59
59
50
38
33
28
27
25
22
20
19
18
18
18
14
13
13
13
11
11
11
11
10
10
10
9
8
7
7
7
7
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus), Affe
1366
797
639
71
56
55
54
44
31
29
6
6
6
5
4
3
3
3
3
1
1
1
1
Wirt
Maus
1063
392
11
4
2
1
Klonalität
Monoklonal
Konjugat
Dieser AKT Antikörper ist konjugiert mit Biotin
55
43
41
40
33
26
15
15
10
10
8
6
6
6
6
6
6
6
6
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Applikation
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
1262
654
603
290
229
206
136
111
61
38
32
26
12
11
10
4
4
3
3
2
2
1
1
1
1
1
1
1
1
Optionen
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Produktnummer ABIN1043752
$ 300.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
11.970353 ABIN1043753 IP IHC ELISA WB Biotin Mouse IgG1 kappa pThr308, Internal Region Anmelden zum Anzeigen 18F3-H11 4
11.970353 ABIN393493 ELISA IF WB Mouse IgG2a AA 1-481 Anmelden zum Anzeigen 1D4 5
11.970353 ABIN349616 IP IHC ELISA WB Mouse IgG1 kappa C-Term Anmelden zum Anzeigen 18F10-E5 0
11.970353 ABIN5596664 ELISA FACS FM IHC WB Biotin Mouse IgG2a Internal Region Anmelden zum Anzeigen 5E5-F5-D7 0
11.970353 ABIN5596663 ELISA FACS FM IHC WB Biotin Mouse IgG2a Internal Region Anmelden zum Anzeigen 14E5-A2-B2-H9 0
11.970353 ABIN5596665 ELISA FACS FM IHC WB FITC Mouse IgG2a Internal Region Anmelden zum Anzeigen 14E5-A2-B2-H9 0
11.970353 ABIN5596666 ELISA FACS FM IHC WB FITC Mouse IgG2a Internal Region Anmelden zum Anzeigen 5E5-F5-D7 0
11.970353 ABIN4880525 ELISA FACS ICC WB Mouse IgG1 AA 1-150 Anmelden zum Anzeigen 1F7E10 0
11.970353 ABIN5596661 ELISA FACS FM IHC WB APC Mouse IgG2a Internal Region Anmelden zum Anzeigen 14E5-A2-B2-H9 0
11.970353 ABIN5596667 ELISA FACS FM IHC WB PE Mouse IgG2a Internal Region Anmelden zum Anzeigen 14E5-A2-B2-H9 0
11.970353 ABIN5542263 ELISA FACS ICC IHC WB Mouse IgG1 AA 1-150 Anmelden zum Anzeigen 1F7E10 0
1 ABIN1043756 FM FRET STED ELISA WB Atto 594 Mouse IgG1 kappa pSer473, Internal Region Anmelden zum Anzeigen 17F6-B11 4
1 ABIN1043730 IP IHC ELISA WB Mouse IgG1 kappa C-Term Anmelden zum Anzeigen 18F10-E5 4
1 ABIN1043729 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Anmelden zum Anzeigen 18F3-H11 4
1 ABIN94783 FM IHC (fp) IP ELISA WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 2
1 ABIN964561 IP IHC ELISA WB Mouse IgG1 kappa pSer473 Anmelden zum Anzeigen 17F6-B11 0
1 ABIN4278999 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pSer473 Anmelden zum Anzeigen 17F6-B11 0
1 ABIN349626 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Anmelden zum Anzeigen 18F3-H11 0
1 ABIN4279000 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pThr308 Anmelden zum Anzeigen 18F3-H11 0
1 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Anmelden zum Anzeigen Polyclonal 3

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antikörper
Epitop pSer473, Internal Region
(235), (117), (113), (59), (59), (50), (38), (33), (28), (27), (25), (22), (20), (19), (18), (18), (18), (14), (13), (13), (13), (11), (11), (11), (11), (10), (10), (10), (9), (8), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus), Affe
(1366), (797), (639), (71), (56), (55), (54), (44), (31), (29), (6), (6), (6), (5), (4), (3), (3), (3), (3), (1), (1), (1), (1)
Wirt Maus
(1063), (392), (11), (4), (2), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser AKT Antikörper ist konjugiert mit Biotin
(55), (43), (41), (40), (33), (26), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Applikation Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(1262), (654), (603), (290), (229), (206), (136), (111), (61), (38), (32), (26), (12), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-AKT Antikörper

Target Details AKT Anwendungsinformationen Handhabung Referenzen für anti-AKT Antikörper (ABIN1043752) Bilder
Spezifität Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
Produktmerkmale AKT phospho 473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Immunogen Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
Immunogen Type: Peptide
Klon 17F6-B11
Isotyp IgG1 kappa
Plasmids, Primers & others

Target Details AKT

Produktdetails anti-AKT Antikörper Anwendungsinformationen Handhabung Referenzen für anti-AKT Antikörper (ABIN1043752) Bilder zurück nach oben
Antigen
Andere Bezeichnung Akt (AKT1 Antibody Abstract)
Hintergrund AKT phospho 473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Synonyms: phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, phospho AKT antibody, Anti-AKT pS473 Antibody Biotin Conjugated
Gen-ID 207, 62241011
UniProt P31749
Pathways PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Anwendungsinformationen

Produktdetails anti-AKT Antikörper Target Details AKT Handhabung Referenzen für anti-AKT Antikörper (ABIN1043752) Bilder zurück nach oben
Applikations-hinweise Biotin Conjugated Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.
Kommentare

Gene Name: AKT1

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-AKT Antikörper Target Details AKT Anwendungsinformationen Referenzen für anti-AKT Antikörper (ABIN1043752) Bilder zurück nach oben
Format Lyophilized
Rekonstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Haltbarkeit 12 months

Referenzen für anti-AKT Antikörper (ABIN1043752)

Produktdetails anti-AKT Antikörper Target Details AKT Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987

Bilder

Produktdetails anti-AKT Antikörper Target Details AKT Anwendungsinformationen Handhabung Referenzen für anti-AKT Antikörper (ABIN1043752) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) Western Blot of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Lane 1: GST t...
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 2)
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 3)
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 4)
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 5)
ELISA image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) ELISA of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Antigen: BSA conjuga...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) Western Blot of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Lane 1: GST t...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) Western Blot of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Lane 1: GST t...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) Immunohistochemistry of mouse anti AKT phospho S473 biotin conjugated at 40X (left) w...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) Immunohistochemistry of mouse Anti-AKT pS473 (MOUSE) Biotin Conjugated  at 40X Tissue...