anti-Maus AKT Antikörper für ELISA

Recommended AKT Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antikörper
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitop
pThr308, Internal Region
230
119
112
58
58
51
36
33
32
25
23
22
21
19
18
18
14
14
13
12
11
11
11
11
10
10
10
9
9
8
7
7
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus), Affe
1328
752
591
63
57
56
54
37
31
28
7
6
6
5
4
3
3
3
1
1
1
1
1
Wirt
Maus
1025
388
11
4
2
1
Klonalität
Monoklonal
Konjugat
Dieser AKT Antikörper ist konjugiert mit Biotin
55
43
43
40
33
26
15
15
10
10
8
6
6
6
6
6
6
6
6
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Applikation
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
1215
616
594
267
203
202
137
90
64
38
32
26
12
11
10
4
4
3
3
2
2
1
1
1
1
1
1
1
Optionen
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Produktnummer ABIN1043753
$ 300.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
10.639014 ABIN1043752 IP IHC ELISA WB Biotin Mouse IgG1 kappa pSer473, Internal Region Anmelden zum Anzeigen 17F6-B11 4
10.639014 ABIN349616 IP IHC ELISA WB Mouse IgG1 kappa C-Term Anmelden zum Anzeigen 18F10-E5 0
10.639014 ABIN5596665 ELISA FACS FM IHC WB FITC Mouse IgG2a Internal Region Anmelden zum Anzeigen 14E5-A2-B2-H9 0
10.639014 ABIN5596666 ELISA FACS FM IHC WB FITC Mouse IgG2a Internal Region Anmelden zum Anzeigen 5E5-F5-D7 0
10.639014 ABIN5596663 ELISA FACS FM IHC WB Biotin Mouse IgG2a Internal Region Anmelden zum Anzeigen 14E5-A2-B2-H9 0
10.639014 ABIN5596664 ELISA FACS FM IHC WB Biotin Mouse IgG2a Internal Region Anmelden zum Anzeigen 5E5-F5-D7 0
10.639014 ABIN5596661 ELISA FACS FM IHC WB APC Mouse IgG2a Internal Region Anmelden zum Anzeigen 14E5-A2-B2-H9 0
10.639014 ABIN5596667 ELISA FACS FM IHC WB PE Mouse IgG2a Internal Region Anmelden zum Anzeigen 14E5-A2-B2-H9 0
10.639014 ABIN5542263 ELISA FACS ICC IHC WB Mouse IgG1 AA 1-150 Anmelden zum Anzeigen 1F7E10 0
1 ABIN94783 FM IHC (fp) IP ELISA WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 2
1 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Anmelden zum Anzeigen Polyclonal 3
1 ABIN1099896 ELISA IF IHC IHC (p) WB Mouse IgG1 pSer473 Anmelden zum Anzeigen 17F6-B11 0
1 ABIN1043756 FM FRET STED ELISA WB Atto 594 Mouse IgG1 kappa pSer473, Internal Region Anmelden zum Anzeigen 17F6-B11 4
1 ABIN1043730 IP IHC ELISA WB Mouse IgG1 kappa C-Term Anmelden zum Anzeigen 18F10-E5 4
1 ABIN1531520 ELISA WB Rabbit IgG AA 292-341, pTyr326 Anmelden zum Anzeigen Polyclonal 2
1 ABIN1043729 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Anmelden zum Anzeigen 18F3-H11 4
1 ABIN1533695 ELISA WB Rabbit IgG AA 11-60 Anmelden zum Anzeigen Polyclonal 1
1 ABIN1531420 ELISA WB Rabbit IgG AA 95-144, pSer129 Anmelden zum Anzeigen Polyclonal 1
1 ABIN1532436 IF IHC ELISA WB Rabbit IgG AA 95-144 Anmelden zum Anzeigen Polyclonal 1
1 ABIN1532434 IHC ELISA Rabbit IgG AA 431-480 Anmelden zum Anzeigen Polyclonal 1

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antikörper
Epitop pThr308, Internal Region
(230), (119), (112), (58), (58), (51), (36), (33), (32), (25), (23), (22), (21), (19), (18), (18), (14), (14), (13), (12), (11), (11), (11), (11), (10), (10), (10), (9), (9), (8), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus), Affe
(1328), (752), (591), (63), (57), (56), (54), (37), (31), (28), (7), (6), (6), (5), (4), (3), (3), (3), (1), (1), (1), (1), (1)
Wirt Maus
(1025), (388), (11), (4), (2), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser AKT Antikörper ist konjugiert mit Biotin
(55), (43), (43), (40), (33), (26), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Applikation Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(1215), (616), (594), (267), (203), (202), (137), (90), (64), (38), (32), (26), (12), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-AKT Antikörper

Target Details AKT Anwendungsinformationen Handhabung Referenzen für anti-AKT Antikörper (ABIN1043753) Bilder
Spezifität Anti-AKT pT308 was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur. Cancer, Cell Signaling, Neuroscience, Signal Transduction research.
Produktmerkmale Anti-AKT phospho T308 is ideal for western blotting, ELISA, IHC and IP. Phospho AKT pT308 antibody is specific for AKT protein phosphorylated at T308. AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. Anti-AKT pT308 monoclonal antibody is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, Signal Transduction research.
Immunogen Anti-AKT pT308 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
Immunogen Type: Peptide
Klon 18F3-H11
Isotyp IgG1 kappa
Plasmids, Primers & others

Target Details AKT

Produktdetails anti-AKT Antikörper Anwendungsinformationen Handhabung Referenzen für anti-AKT Antikörper (ABIN1043753) Bilder zurück nach oben
Antigen
Andere Bezeichnung Akt (AKT1 Antibody Abstract)
Hintergrund Anti-AKT phospho T308 is ideal for western blotting, ELISA, IHC and IP. Phospho AKT pT308 antibody is specific for AKT protein phosphorylated at T308. AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. Anti-AKT pT308 monoclonal antibody is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, Signal Transduction research.
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, Akt phospho T308 Antibody, Anti-AKT pT308 Monoclonal Antibody Biotin Conjugated
Gen-ID 207, 62241011
UniProt P31749
Pathways PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Anwendungsinformationen

Produktdetails anti-AKT Antikörper Target Details AKT Handhabung Referenzen für anti-AKT Antikörper (ABIN1043753) Bilder zurück nach oben
Applikations-hinweise Biotin Conjugated Anti-AKT pT308 is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pT308 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.
Kommentare

Gene Name: AKT1

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-AKT Antikörper Target Details AKT Anwendungsinformationen Referenzen für anti-AKT Antikörper (ABIN1043753) Bilder zurück nach oben
Format Lyophilized
Rekonstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Haltbarkeit 12 months

Referenzen für anti-AKT Antikörper (ABIN1043753)

Produktdetails anti-AKT Antikörper Target Details AKT Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987

Bilder

Produktdetails anti-AKT Antikörper Target Details AKT Anwendungsinformationen Handhabung Referenzen für anti-AKT Antikörper (ABIN1043753) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (ABIN1043753) Western Blot of Mouse anti-Akt phospho T308 Biotin Conjugated antibody. Lane 1: GST t...
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (ABIN1043753) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (Image 2)
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (ABIN1043753) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (Image 3)
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (ABIN1043753) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (Image 4)
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (ABIN1043753) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (Image 5)
ELISA image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (ABIN1043753) ELISA of Mouse anti-Akt phospho T308 Biotin Conjugated antibody. Antigen: Unconjugate...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (ABIN1043753) Western Blot of Mouse anti-Akt phospho T308 Biotin Conjugated antibody. Lane 1: GST t...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (ABIN1043753) Western Blot of Mouse anti-Akt phospho T308 Biotin Conjugated antibody. Lane 1: GST t...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pThr308) antibody (Biotin) (ABIN1043753) Immunohistochemistry of Mouse Anti-AKT phospho T308 Biotin Conjugated at 20X (left) a...