anti-Ratte (Rattus) CHEK1 Antikörper für Immunofluorescence (Paraffin-embedded Sections)

Recommended CHEK1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Checkpoint Kinase 1 (CHEK1) Antikörper
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CAMK/CAMKL/CHK1 protein kinase Chk1
  • checkpoint kinase 1 S homeolog
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1.S
  • Chek1
  • chek1
Epitop
AA 280-330, pSer317
68
58
39
35
29
17
16
14
14
13
7
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
500
205
176
14
14
10
10
10
7
5
4
4
4
2
1
1
Wirt
Kaninchen
441
52
22
6
Klonalität
Polyklonal
Konjugat
Dieser CHEK1 Antikörper ist unkonjugiert
14
13
12
8
8
8
3
3
3
3
3
3
3
3
3
3
3
2
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
436
216
173
81
70
39
33
21
18
16
7
7
6
6
Optionen
Hersteller
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Bilder

Produktnummer ABIN757072
$ 340.45
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN757075 IF (p) Cy3 Rabbit IgG AA 280-330, pSer317 Anmelden zum Anzeigen Polyclonal 0
1 ABIN757078 IF (p) Cy7 Rabbit IgG AA 280-330, pSer317 Anmelden zum Anzeigen Polyclonal 0
1 ABIN757079 IF (p) FITC Rabbit IgG AA 280-330, pSer317 Anmelden zum Anzeigen Polyclonal 0
1 ABIN757076 IF (p) Cy5 Rabbit IgG AA 280-330, pSer317 Anmelden zum Anzeigen Polyclonal 0
1 ABIN757077 IF (p) Cy5.5 Rabbit IgG AA 280-330, pSer317 Anmelden zum Anzeigen Polyclonal 0
1 ABIN875500 IF (p) Alexa Fluor 647 Rabbit IgG AA 280-330, pSer317 Anmelden zum Anzeigen Polyclonal 0
1 ABIN875501 IF (p) Alexa Fluor 350 Rabbit IgG AA 280-330, pSer317 Anmelden zum Anzeigen Polyclonal 0
1 ABIN875499 IF (p) Alexa Fluor 555 Rabbit IgG AA 280-330, pSer317 Anmelden zum Anzeigen Polyclonal 0
1 ABIN875498 IF (p) Alexa Fluor 488 Rabbit IgG AA 280-330, pSer317 Anmelden zum Anzeigen Polyclonal 0
1 ABIN2811603 IF (p) Alexa Fluor 594 Rabbit IgG AA 280-330, pSer317 Anmelden zum Anzeigen Polyclonal 0
1 ABIN757484 IF (p) FITC Rabbit IgG pSer345 Anmelden zum Anzeigen Polyclonal 0
1 ABIN757481 IF (p) Cy5 Rabbit IgG pSer345 Anmelden zum Anzeigen Polyclonal 0
1 ABIN757483 IF (p) Cy7 Rabbit IgG pSer345 Anmelden zum Anzeigen Polyclonal 0
1 ABIN757480 IF (p) Cy3 Rabbit IgG pSer345 Anmelden zum Anzeigen Polyclonal 0
1 ABIN757482 IF (p) Cy5.5 Rabbit IgG pSer345 Anmelden zum Anzeigen Polyclonal 0
1 ABIN875675 IF (p) Alexa Fluor 555 Rabbit IgG pSer345 Anmelden zum Anzeigen Polyclonal 0
1 ABIN875671 IF (p) Alexa Fluor 647 Rabbit IgG pSer345 Anmelden zum Anzeigen Polyclonal 0
1 ABIN875673 IF (p) Alexa Fluor 488 Rabbit IgG pSer345 Anmelden zum Anzeigen Polyclonal 0
1 ABIN875674 IF (p) Alexa Fluor 350 Rabbit IgG pSer345 Anmelden zum Anzeigen Polyclonal 0
1 ABIN4999968 IF (p) Alexa Fluor 680 Rabbit IgG AA 280-330, pSer317 Anmelden zum Anzeigen Polyclonal 0

General

Antigen Checkpoint Kinase 1 (CHEK1) Antikörper
Epitop AA 280-330, pSer317
(68), (58), (39), (35), (29), (17), (16), (14), (14), (13), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(500), (205), (176), (14), (14), (10), (10), (10), (7), (5), (4), (4), (4), (2), (1), (1)
Wirt Kaninchen
(441), (52), (22), (6)
Klonalität
Polyklonal
Konjugat Dieser CHEK1 Antikörper ist unkonjugiert
(14), (13), (12), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(436), (216), (173), (81), (70), (39), (33), (21), (18), (16), (7), (7), (6), (6)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-CHEK1 Antikörper

Target Details CHEK1 Anwendungsinformationen Handhabung Referenzen für anti-CHEK1 Antikörper (ABIN757072) Bilder
Spezifität This phosphorylation site is homologous to that of Ser317 in Mouse and Rat.
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human CHEK1 around the phosphorylation site of Ser317
Isotyp IgG
Plasmids, Primers & others

Target Details CHEK1

Produktdetails anti-CHEK1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-CHEK1 Antikörper (ABIN757072) Bilder zurück nach oben
Antigen
Andere Bezeichnung Chek1 (CHEK1 Antibody Abstract)
Hintergrund

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA.

Subcellular location: Cytoplasm, Nucleus

Synonyms: CHK1, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1

Gen-ID 1111
UniProt O14757
Pathways p53 Signalweg, Apoptose, Zellzyklus

Anwendungsinformationen

Produktdetails anti-CHEK1 Antikörper Target Details CHEK1 Handhabung Referenzen für anti-CHEK1 Antikörper (ABIN757072) Bilder zurück nach oben
Applikations-hinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-CHEK1 Antikörper Target Details CHEK1 Anwendungsinformationen Referenzen für anti-CHEK1 Antikörper (ABIN757072) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Referenzen für anti-CHEK1 Antikörper (ABIN757072)

Produktdetails anti-CHEK1 Antikörper Target Details CHEK1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Guo, Cui, Peng, Fang, Zuo, Deng, Wang, Wu, Chen, Deng: "Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney." in: Oncotarget, Vol. 6, Issue 34, pp. 35964-77, 2015 Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Chicken). Weitere Details: Sample: kidney

Bilder

Produktdetails anti-CHEK1 Antikörper Target Details CHEK1 Anwendungsinformationen Handhabung Referenzen für anti-CHEK1 Antikörper (ABIN757072) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 280-330), (pSer317) antibody (ABIN757072) Formalin-fixed and paraffin embedded mouse small intestine tissue labeled with Anti-p...
SDS-PAGE (SDS) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 280-330), (pSer317) antibody (ABIN757072) Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti phospho-CHEK1(Se...