anti-Human Insulin Antikörper für Immunoprecipitation

Recommended Insulin Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Insulin (INS) Antikörper
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reaktivität
Human, Schwein
952
468
370
350
240
194
45
11
9
8
7
7
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Wirt
Maus
785
197
49
9
4
2
1
Klonalität
Monoklonal
Konjugat
Dieser Insulin Antikörper ist unkonjugiert
52
41
36
21
20
20
20
15
15
15
13
13
13
13
13
13
13
10
9
9
7
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
461
358
276
269
259
236
180
118
106
66
66
31
25
23
21
8
7
5
4
4
4
3
2
2
2
1
1
1
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN5556681
$ 619.30
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN129507 IP IHC ELISA WB Mouse IgG Anmelden zum Anzeigen 2D11-H5 0
1 ABIN1107760 IHC (p) IP Mouse IgG1 Anmelden zum Anzeigen ISL-8J 1
1 ABIN4326102 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Anmelden zum Anzeigen SPM139 0
1 ABIN4326030 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Anmelden zum Anzeigen SPM139 0
1 ABIN2742155 ELISA FACS IF IHC IHC (p) IP WB Mouse IgG1, kappa AA 1-84 Anmelden zum Anzeigen 2D11-H5 or INS05 0
1 ABIN4326104 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Anmelden zum Anzeigen SPM139 0
1 ABIN4326111 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Anmelden zum Anzeigen SPM139 0
1 ABIN4326112 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Anmelden zum Anzeigen SPM139 0
1 ABIN4326114 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Anmelden zum Anzeigen SPM139 0
1 ABIN4326115 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Anmelden zum Anzeigen SPM139 0
1 ABIN4326055 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Anmelden zum Anzeigen E2-E3 (same as INS04) 0
1 ABIN4326058 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Anmelden zum Anzeigen E2-E3-2D11-H5 (same as INS04-INS05) 0
1 ABIN4326033 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Anmelden zum Anzeigen SPM531 0
1 ABIN4326109 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Anmelden zum Anzeigen SPM139 0
1 ABIN4326064 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Anmelden zum Anzeigen SPM139 0
1 ABIN2742156 ELISA FACS IF IHC IHC (p) IP WB Mouse IgG1, kappa AA 1-84 Anmelden zum Anzeigen E2-E3 or INS04 0
1 ABIN2742157 FACS IF IHC IHC (p) IP WB Mouse IgG1, kappa AA 1-84 Anmelden zum Anzeigen E2-E3-2D11-H5 or INS04-INS05 0
1 ABIN4904069 IF IHC IP Mouse IgG Anmelden zum Anzeigen 0
1 ABIN2294343 FACS IF IP IHC ELISA WB Mouse IgG1 kappa AA 1-84 Anmelden zum Anzeigen 2D11-H5 0
1 ABIN4326118 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Anmelden zum Anzeigen SPM531 0

General

Antigen Insulin (INS) Antikörper
Reaktivität Human, Schwein
(952), (468), (370), (350), (240), (194), (45), (11), (9), (8), (7), (7), (6), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Wirt Maus
(785), (197), (49), (9), (4), (2), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser Insulin Antikörper ist unkonjugiert
(52), (41), (36), (21), (20), (20), (20), (15), (15), (15), (13), (13), (13), (13), (13), (13), (13), (10), (9), (9), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1)
Applikation Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(461), (358), (276), (269), (259), (236), (180), (118), (106), (66), (66), (31), (25), (23), (21), (8), (7), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Insulin Antikörper

Target Details Insulin Anwendungsinformationen Handhabung Bilder
Sequenz Human Proinsulin (precursor) 21 aa A chain 90-110. 30 aa B chain 25-54. 1 MALWMRLLPL LALLALWGPD PAAAFVNQHL CGSHLVEALY LVCGERGFFY TPKTRREAED 61 LQVGQVELGG GPGAGSLQPL ALEGSLQKRG IVEQCCTSIC SLYQLENYCN
Spezifität This monoclonal 2D11.H5 antibody recognizes a polypeptide which is identified as Insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. This protein A purified Mouse monoclonal antibody reacts specifically with Insulin from Human and Swine sources. Cross reactivity with Insulin from Mouse and Rat does not occur. Cross reactivity with Insulin from other sources has not been determined.
Cellular Localization: Cytoplasmic.Cellular Localization:
Produktmerkmale Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)
Reinigung Protein A Chromatography
Immunogen Purified human Insulin coupled to Bovine Serum Albumin (BSA).
Klon 2D11-H5
Isotyp IgG1
Plasmids, Primers & others

Target Details Insulin

Produktdetails anti-Insulin Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Insulin (INS Antibody Abstract)
Hintergrund Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-peptide basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain acids. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as β-cell and tumor (insulinoma) markers.Synonyms: INS
Molekulargewicht 6 kDa
Gen-ID 3630
NCBI Accession NP_000198
UniProt P01308
Pathways NF-kappaB Signalweg, RTK Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagie, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Anwendungsinformationen

Produktdetails anti-Insulin Antikörper Target Details Insulin Handhabung Bilder zurück nach oben
Applikations-hinweise This antibody is suitable for ELISA, Immunohistochemistry, Immunoblotting andimmunoprecipitation. Insulin has a cytoplasmic localization. Pancreatic tissue or β-cellsfrom islets of Langerhans can be used as a positive control. Generally a 1: 50 to 1: 200 dilution is suitable for most applications. A 30 to 60 minuteincubation time is usually sufficient. However, depending upon the fixation conditions andthe staining system employed, optimal incubation should be determined by the user. Frozen sections of paraffin embedded tissues are recommended for preparations to use forimmunohistochemistry. No special pretreatment is required. Researchers shoulddetermine the optimal dilution for other specific assays and cell types. ELISA: Results of Mab anti-Insulin antibody were tested against human insulin by ELISA. Each well was coated with 0.1g of conjugate. The starting concentration of antibody in thedilution series was 10 μg/mL. Each point on the Y-axis represents a 3-fold dilution. Themidpoint of the titration curve represents approx-imately 5 ng/mL antibody or a 1: 200,000dilution from the stock concentration. HRP conjugated Gt-a- Mouse IgG H&L and TMBsubstrate were used for detection (see figure 1).
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Insulin Antikörper Target Details Insulin Anwendungsinformationen Bilder zurück nach oben
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.