Multiple Tag Cell Lysate

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Proteinspezies
Escherichia coli (E. coli)
Lysatspezies
Human Cells
Applikation
Dot Blot (DB), Western Blotting (WB)
Optionen
Hersteller
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Lysate Fraction Whole Cell Lysate
Lysate Type Overexpression Lysate
Lysed Cells HEK 293T Cells
Hintergrund Epitope Tag Positive Control Lysates. Multiple Tag Cell Lysate is whole E. coli cell lysate comprised of about 30% specially designed fusion protein, which act as a positive control for Western blot or Dot blot. The fusion protein is a 52 kD protein containing 16 commonly used tags, which are, in order, Trx, T7, HSV, C-Myc, SVS-g, Glu-Glu, V5, E tag, DYKDDDDK, S-tag, HA, KT3, E2, AU1, AU5, and His tag.
Applikations-hinweise Positive control for Western blot and Dot blot.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 200 µg of whole E. coli cell lysate (lyophilized) Volume Add 200 µl of ddH2O to make the final of 1 µg/µl in 1X SDS Loading Buffer. 0.02 - 0.5 µl of the lysate is recommended for each lane of a minigel. For accurate loading, dilute with 1X SDS Loading Buf
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C or below. After reconstitution, aliquot and store at -20°C or below.
Produkt verwendet in: Lawless, Mankan, White, ODwyer, Norris: "Expression of hereditary hemochromatosis C282Y HFE protein in HEK293 cells activates specific endoplasmic reticulum stress responses." in: BMC cell biology, Vol. 8, pp. 30, 2007 (PubMed).