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DYKDDDDK Positive Control Lysate

PC, WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Tag
Produktnummer ABIN1607004
  • Target Alle DYKDDDDK Tag Produkte
    DYKDDDDK Tag
    Proteinspezies
    Tag
    Lysatspezies
    Human Cells
    Applikation
    Positive Control (PC), Western Blotting (WB)
    Endotoxin-Niveau
    Low Endotoxin : No
    Lysed Cells
    HEK 293T Cells
    Lysate Type
    Overexpression Lysate
    Lysate Fraction
    Whole Cell Lysate
  • Applikationshinweise
    Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). If dissociating conditions are desired, add reducing agent prior to heating. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for SDS-PAGE. The product is supplied in 1X loading buffer and is ready-to-use.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
    Haltbarkeit
    Expiration date is three (3) months from date of opening.
  • Target
    DYKDDDDK Tag
    Abstract
    DYKDDDDK Tag Produkte
    Substanzklasse
    Tag
    Hintergrund
    This product is intended as a positive control for use with anti-DYKDDDDK tag antibodies.
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