DYKDDDDK Positive Control Lysate

Details zu Produkt Nr. ABIN1607004
Protein
Proteinspezies
Tag
Lysatspezies
Human Cells
Applikation
Positive Control (PC), Western Blotting (WB)
Optionen
Endotoxin-Niveau Low Endotoxin : No
Lysate Fraction Whole Cell Lysate
Lysate Type Overexpression Lysate
Lysed Cells HEK 293T Cells
Protein
Substanzklasse Tag
Hintergrund This product is intended as a positive control for use with anti-DYKDDDDK tag antibodies.
Applikations-hinweise Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). If dissociating conditions are desired, add reducing agent prior to heating. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for SDS-PAGE. The product is supplied in 1X loading buffer and is ready-to-use.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
Lagerung -80 °C
Informationen zur Lagerung Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
Haltbarkeit Expiration date is three (3) months from date of opening.
Bilder des Herstellers
Western Blotting (WB) image for DYKDDDDK Positive Control Lysate (ABIN1607004) Western Blot of DYKDDDDK (FLAG®) Positive Control Lysate. Lane 1: DYKDDDDK (FLAG®) Po...
Western Blotting (WB) image for DYKDDDDK Positive Control Lysate (ABIN1607004) DYKDDDDK (FLAG®) Positive Control Lysate Western Blot of DYKDDDDK (FLAG®) Positive Co...