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DCX HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

DCX WB, IP Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
Produktnummer ABIN1339650
  • Target Alle Doublecortin (DCX) Produkte
    Doublecortin (DCX)
    Proteinspezies
    Human
    Lysatspezies
    Human Cells
    Applikation
    Western Blotting (WB), Immunoprecipitation (IP)
    Verwendungszweck
    Transient Overexpression Lysate
    Spezifität
    Non-denatured
    Produktmerkmale
    DCX HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
    Used Plasmid: pCMV-DCX full length
    Lysed Cells
    HEK 293T Cells
    Lysate Type
    Overexpression Lysate
    Lysate Fraction
    Whole Cell Lysate
  • Applikationshinweise
    Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
    Kommentare

    Product Quality tested by: Transient overexpression cell lysate was tested with Anti-DCX antibody by Western Blots.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    2 mg/mL
    Buffer
    Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
    Handhabung
    Aliquot to avoid repeated freezing and thawing.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
  • Target
    Doublecortin (DCX)
    Andere Bezeichnung
    DCX (DCX Produkte)
    Synonyme
    DCX Lysate, DBCN Lysate, DC Lysate, LISX Lysate, SCLH Lysate, XLIS Lysate, Dbct Lysate, 18C15.5 Lysate, doublecortin Lysate, DCX Lysate, Dcx Lysate
    Hintergrund
    Full Gene Name: doublecortin
    Synonyms: DBCN,DC,LISX,SCLH,XLIS
    Gen-ID
    1641
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