ERK2 ELISA Kit

Produktnummer ABIN8083671

Das Human, Ratte und Maus ERK2 ELISA Kit (ABIN8083671) ist ein Colorimetric ELISA Kit zur Detektion von Human, Ratte und Maus ERK2.

Kurzübersicht für ERK2 ELISA Kit (ABIN8083671)

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reaktivität: Human, Ratte, Maus

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Culture Cells

Produktdetails ERK2 ELISA Kit

Verwendungszweck: p42 MAPK Colorimetric Cell-Based ELISA for detecting p42 MAPK in researchers plate their cell line of choice.

Analytische Methode: Qualitative

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Testdauer: 4 - 5 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 6 months

Details zu ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Andere Bezeichnung: p42 MAPK

Hintergrund: Gene Aliases: ERK-2, ERK2, ERT1, Extracellular signal- regulated kinase 2, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK, MAPK 2, MAPK1, MK01, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, P42-MAPK, PRKM1, kinase ERK2, p42-MAPK

Gen-ID: 5594

UniProt: P28482

Pathways: MAPK Signalweg, RTK Signalweg, Apoptose, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagie, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteine