IKK alpha ELISA Kit

Produktnummer ABIN7830598

Human IKK alpha ELISA Kit, Colorimetric Assay für die Quantifizierung von Human IKK alpha.

Kurzübersicht für IKK alpha ELISA Kit (ABIN7830598)

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails IKK alpha ELISA Kit

Verwendungszweck: CHUK/IKBKB (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CHUK/IKBKB expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu IKK alpha

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Andere Bezeichnung: CHUK

Hintergrund:

Synonyms: IKBKA|IKK-alpha|IKK1|IKKA|NFKBIKA|TCF16

Gene Description: conserved helix-loop-helix ubiquitous kinase

Gen-ID: 1147

UniProt: O15111

Pathways: PI3K-Akt Signalweg, NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteine