RAD51 ELISA Kit

Produktnummer ABIN6951284

Produktdetails RAD51 ELISA Kit

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human RAD51 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Andere Bezeichnung: RAD51 (RAD51 Produkte)

Synonyme: BRCC5 ELISA Kit, HRAD51 ELISA Kit, HsRad51 ELISA Kit, HsT16930 ELISA Kit, MRMV2 ELISA Kit, RAD51A ELISA Kit, RECA ELISA Kit, CRAD51 ELISA Kit, wu:fb38e12 ELISA Kit, zgc:77754 ELISA Kit, RGD1563603 ELISA Kit, ATRAD51 ELISA Kit, F22D1.20 ELISA Kit, F22D1_20 ELISA Kit, RAS associated with diabetes protein 51 ELISA Kit, AV304093 ELISA Kit, Rad51a ELISA Kit, Reca ELISA Kit, brcc5 ELISA Kit, rad51 ELISA Kit, rad51a ELISA Kit, reca ELISA Kit, xrad51 ELISA Kit, RAD51 recombinase ELISA Kit, RAS associated with diabetes protein 51 ELISA Kit, RAD51 recombinase L homeolog ELISA Kit, RAD51 ELISA Kit, rad51 ELISA Kit, Rad51 ELISA Kit, rad51.L ELISA Kit

Gen-ID: 5888

UniProt: Q06609

Pathways: DNA Reparatur