TRKB ELISA Kit

Produktnummer ABIN6951196

Produktdetails TRKB ELISA Kit

Target: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Bindungsspezifität: pTyr816

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Phospho-TrkB (Tyr816) and Total TrkB ELISA Kit. This assay semi-quantitatively measures TrkB phosphorylated at Tyrosine-816 as well as total TrkB in lysate samples.

Proben: Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: This ELISA kit recognizes Human TrkB phosphorylated at Tyrosine-816 and Total TrkB.

Produktmerkmale:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu TRKB

Target: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Andere Bezeichnung: TrkB (NTRK2 Produkte)

Synonyme: trkb ELISA Kit, trkb-b ELISA Kit, xtrkb ELISA Kit, si:ch211-220b11.1 ELISA Kit, trkB2 ELISA Kit, NTRK2 ELISA Kit, ntrk2 ELISA Kit, gp145-trkb ELISA Kit, TRKB ELISA Kit, GP145-TrkB ELISA Kit, trk-B ELISA Kit, AI848316 ELISA Kit, C030027L06Rik ELISA Kit, Tkrb ELISA Kit, trkB ELISA Kit, RATTRKB1 ELISA Kit, TRKB1 ELISA Kit, Trk-B ELISA Kit, TrkB ELISA Kit, neurotrophic receptor tyrosine kinase 2 ELISA Kit, neurotrophic receptor tyrosine kinase 2 L homeolog ELISA Kit, neurotrophic tyrosine kinase, receptor, type 2b ELISA Kit, neurotrophic receptor tyrosine kinase 2 S homeolog ELISA Kit, neurotrophic tyrosine kinase, receptor, type 2 ELISA Kit, NTRK2 ELISA Kit, ntrk2.L ELISA Kit, ntrk2b ELISA Kit, ntrk2.S ELISA Kit, ntrk2 ELISA Kit, Ntrk2 ELISA Kit

Gen-ID: 4915

UniProt: P15209

Pathways: RTK Signalweg, Neurotrophin Signalübertragung, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport