WNT3A ELISA Kit
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- Target Alle WNT3A ELISA Kits anzeigen
- WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
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Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Applikation
- ELISA
- Verwendungszweck
- Mouse Wnt-3a ELISA Kit.
- Proben
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytische Methode
- Quantitative
- Spezifität
- This ELISA antibody pair recognizes Mouse Wnt-3a.
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product WNT3A ELISA Kit
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle WNT3A ELISA Kits anzeigen
- WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
- Andere Bezeichnung
- Wnt-3a (WNT3A Produkte)
- Synonyme
- WNT3A ELISA Kit, Wnt-3a ELISA Kit, vt ELISA Kit, Zwnt[a] ELISA Kit, wnt3 ELISA Kit, wnt3 l ELISA Kit, wnt3l ELISA Kit, wnt[a] ELISA Kit, Xwnt-3a ELISA Kit, wnt-3a ELISA Kit, wnt3a-A ELISA Kit, xwnt3a ELISA Kit, WNT-3A ELISA Kit, WNT3 ELISA Kit, wingless-type MMTV integration site family, member 3A ELISA Kit, Wnt family member 3A ELISA Kit, wingless-type MMTV integration site family member 3A L homeolog ELISA Kit, WNT3A ELISA Kit, Wnt3a ELISA Kit, wnt3a ELISA Kit, wnt3a.L ELISA Kit
- UniProt
- P27467
- Pathways
- WNT Signalweg, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity
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