ADAMTS4 ELISA Kit

Produktnummer ABIN6720258

Produktdetails ADAMTS4 ELISA Kit

Target: ADAMTS4 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 4 (ADAMTS4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 625 pg/mL - 40000 pg/mL

Untere Nachweisgrenze: 625 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 4, A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4, A disintegrin like and metalloprotease with thrombospondin type 1 motif 4, ADAM metallopeptidase with thrombospondin type 1 motif 4, ADAM TS 4, ADAM TS4, ADAM-TS 4, ADAM-TS4, ADAMTS2, ADAMTS4, ADAMTS4 protein, ADMP 1, ADMP-1, ADMP1, Aggrecanase1, ATS4_HUMAN, KIAA0688

Background: ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.

Gene Name: ADAMTS4

Production: Natural and recombinant human ADAMTS4. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: CHO, Immunogen sequence: F213-C685

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human ADAMTS4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ADAMTS4 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 625pg/mL-40,000pg/mL

Other Performance Data: DOE: 2-8° C - 09/16/18 -20° C - 03/19/19

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu ADAMTS4

Target: ADAMTS4 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 4 (ADAMTS4))

Andere Bezeichnung: ADAMTS4 (ADAMTS4 Produkte)

Synonyme: 3830423K05 ELISA Kit, ADAM-TS4 ELISA Kit, ADAMTS-2 ELISA Kit, ADMP-1 ELISA Kit, mKIAA0688 ELISA Kit, ADAMTS-4 ELISA Kit, ADAMTS4 ELISA Kit, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif 4 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif, 4 ELISA Kit, Adamts4 ELISA Kit, ADAMTS4 ELISA Kit

Gen-ID: 9507

NCBI Accession: NP_001307265

UniProt: O75173