CD137 ELISA Kit

Produktnummer ABIN6720180

Produktdetails CD137 ELISA Kit

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: 4-1BB, 4-1BB ligand receptor, CD137, CD137 antigen, CDw137, Homolog of mouse 4 1BB, ILA, Induced by lymphocyte activation (ILA), Interleukin activated receptor homolog of mouse Ly63, Receptor protein 4 1BB, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, TNR9_HUMAN, Tumor necrosis factor receptor superfamily member 9

Background: CD137 is a member of the tumor necrosis factor(TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9(TNFRSF9), 4-1BB and induced by lymphocyte activation(ILA). CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.

Gene Name: TNFRSF9

Production: Natural and recombinant human TNFRSF9. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: L24-Q186

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TNFRSF9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TNFRSF9 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu CD137

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Andere Bezeichnung: TNFRSF9 - 4-1BB (TNFRSF9 Produkte)

Synonyme: TNFRSF9 ELISA Kit, zgc:136557 ELISA Kit, 4-1BB ELISA Kit, CD137 ELISA Kit, CDw137 ELISA Kit, ILA ELISA Kit, A930040I11Rik ELISA Kit, AA408498 ELISA Kit, AI325004 ELISA Kit, Cd137 ELISA Kit, Ly63 ELISA Kit, tumor necrosis factor receptor superfamily member 9 ELISA Kit, TNF receptor superfamily member 9 ELISA Kit, tumor necrosis factor receptor superfamily, member 9a ELISA Kit, tumor necrosis factor receptor superfamily, member 9 ELISA Kit, TNFRSF9 ELISA Kit, tnfrsf9a ELISA Kit, Tnfrsf9 ELISA Kit

Gen-ID: 3604

NCBI Accession: NP_001552

UniProt: Q07011

Pathways: Cancer Immune Checkpoints