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HGF ELISA Kit
HGF
Reaktivität: Human
Colorimetric
Sandwich ELISA
3-2000 pg/mL
Cell Lysate, Tissue Lysate
Produktdetails HGF ELISA Kit
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Target
Alle HGF ELISA Kits anzeigen
HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
Reaktivität
Alle Reaktivitäten für HGF ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
3-2000 pg/mL
Untere Nachweisgrenze
3 pg/mL
Applikation
ELISA
Verwendungszweck
Human HGF ELISA Kit for cell and tissue lysate samples.
Proben
Tissue Lysate, Cell Lysate
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes human HGF.
Sensitivität
3 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis Cell lysate buffer
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu HGF
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Target
Alle HGF ELISA Kits anzeigen
HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
Andere Bezeichnung
HGF (HGF Produkte )
Synonyme
DFNB39 ELISA Kit, F-TCF ELISA Kit, HGFB ELISA Kit, HPTA ELISA Kit, SF ELISA Kit, HGF ELISA Kit, C230052L06Rik ELISA Kit, HGF/SF ELISA Kit, NK1 ELISA Kit, NK2 ELISA Kit, SF/HGF ELISA Kit, hepatocyte growth factor ELISA Kit, HGF ELISA Kit, Hgf ELISA Kit
Hintergrund
Gene Names: HGF HPTA Protein names: Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain]
Gen-ID
3082
UniProt
P14210
Pathways
RTK Signalweg , Carbohydrate Homeostasis , Glycosaminoglycan Metabolic Process , Synaptic Membrane , Signaling of Hepatocyte Growth Factor Receptor
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