IKK alpha FLISA Kit

Produktnummer ABIN5691968

Das Human, Maus und Ratte IKK alpha ELISA Kit (ABIN5691968) ist ein Fluorometric ELISA Kit zur Detektion von Human, Maus und Ratte IKK alpha.

Kurzübersicht für IKK alpha FLISA Kit (ABIN5691968)

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Bindungsspezifität: pSer176, pSer177

Reaktivität: Human, Maus, Ratte

Nachweismethode: Fluorometric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Culture Cells

Produktdetails IKK alpha FLISA Kit

Verwendungszweck: The CytoFluor™ Phospho Cell-Based ELISAs provides a convenient method for high-throughput screening of the effects of various drugs, metabolites and various treatment effects on protein expression and phosphorylation profiles in whole cells.

Analytische Methode: Qualitative

Produktmerkmale: Detection of proteins from pg/mL to ng/mL concentrations
- Convenient and ready-to-use
- High-sensitivity, low background with exceptional signal-to-noise ratio
- 450 nm colorimetric detection
- Human, mouse and rat targets
- For use with cell lysates, supernatants, sera and plasma

Bestandteile: 2 x 96-Well Tissue Culture Microplates
100x Anti-Phospho Target Primary Antibody
100x Anti-Target Primary Antibody
100x Anti-Control Primary Antibody
Dye 1-Conjugated Anti-Rabbit IgG Secondary Antibody
Dye 2-Conjugated Anti-Mouse IgG Secondary Antibody
10x TBS
15x Wash Buffer
Quenching Buffer
Blocking Buffer
Primary Antibody Diluent
SDS Solution
Adhesive Plate Seals
Technical Manual

Anwendungsinformationen

Applikationshinweise: Detection Range: At least 5000 cells/well

Testdauer: 4.5 h

Plattentyp: Uncoated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C/6 Months

Haltbarkeit: 6 months

Antigendetails

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Andere Bezeichnung: IKK-alpha

UniProt: O15111, O14920

Pathways: PI3K-Akt Signalweg, NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteine