COL3A1 ELISA Kit

Produktnummer ABIN5674676

Produktdetails COL3A1 ELISA Kit

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.25 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 14.5 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu COL3A1

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Andere Bezeichnung: Collagen Type III Alpha 1 (COL3a1) (COL3A1 Produkte)

Synonyme: EDS4A ELISA Kit, AW550625 ELISA Kit, Col3a-1 ELISA Kit, MMS10-W ELISA Kit, Ms10w ELISA Kit, mKIAA4231 ELISA Kit, col3a1 ELISA Kit, collagen type III alpha 1 chain ELISA Kit, collagen, type III, alpha 1 ELISA Kit, collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant) S homeolog ELISA Kit, COL3A1 ELISA Kit, Col3a1 ELISA Kit, col3a1 ELISA Kit, col3a1.S ELISA Kit

Hintergrund: Alternative name: COL3-A1, COL3A1, EDS4A, Collagen Type III Alpha 1, Ehlers-Danlos Syndrome Type IV Autosomal Dominant, Collagen Alpha-1(III)chain

Gen-ID: 12825

UniProt: P08121

Pathways: Autophagie, Growth Factor Binding