FLT1 CLIA Kit

Produktnummer ABIN5659881

Produktdetails FLT1 CLIA Kit

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12 pg/mL - 20000 pg/mL

Untere Nachweisgrenze: 78.12 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor Receptor 1 (VEGFR1). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) and analogues was observed.

Sensitivität: 9.9 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor Receptor 1 (VEGFR1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor Receptor 1 (VEGFR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Andere Bezeichnung: Vascular Endothelial Growth Factor Receptor 1 (FLT1 Produkte)

Synonyme: FLT CLIA Kit, FLT-1 CLIA Kit, VEGFR-1 CLIA Kit, VEGFR1 CLIA Kit, FLT1 CLIA Kit, flt1b CLIA Kit, sFlt1 CLIA Kit, AI323757 CLIA Kit, Flt-1 CLIA Kit, fms related tyrosine kinase 1 CLIA Kit, FMS-related tyrosine kinase 1 CLIA Kit, vascular endothelial growth factor receptor 1 CLIA Kit, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) CLIA Kit, FMS-like tyrosine kinase 1 CLIA Kit, vascular endothelial growth factor receptor-1 CLIA Kit, FLT1 CLIA Kit, Flt1 CLIA Kit, CpipJ_CPIJ012087 CLIA Kit, flt1 CLIA Kit, FLT-1 CLIA Kit

Hintergrund:

Gene Name: Vascular Endothelial Growth Factor Receptor 1

Gene Aliases: Flt1, Fms-Related Tyrosine Kinase 1, Vascular Permeability Factor Receptor, Fms-like tyrosine kinase 1, Tyrosine-protein kinase receptor FLT

Gen-ID: 14254

UniProt: P35969

Pathways: RTK Signalweg, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals