CXCL1 CLIA Kit

Produktnummer ABIN5657356

Produktdetails CXCL1 CLIA Kit

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Neutrophil Activating Protein 3 (NAP3). No significant cross-reactivity or interference between Neutrophil Activating Protein 3 (NAP3) and analogues was observed.

Sensitivität: 0.59 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Neutrophil Activating Protein 3 (NAP3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Neutrophil Activating Protein 3 (NAP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Neutrophil Activating Protein 3 (NAP3) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil Activating Protein 3 (NAP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil Activating Protein 3 (NAP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Andere Bezeichnung: Neutrophil Activating Protein 3 (CXCL1 Produkte)

Synonyme: FSP CLIA Kit, GRO1 CLIA Kit, GROa CLIA Kit, MGSA CLIA Kit, MGSA-a CLIA Kit, NAP-3 CLIA Kit, SCYB1 CLIA Kit, CINC-1 CLIA Kit, Gro1 CLIA Kit, CXCL1 CLIA Kit, Sdf1 CLIA Kit, Fsp CLIA Kit, KC CLIA Kit, Mgsa CLIA Kit, N51 CLIA Kit, Scyb1 CLIA Kit, gro CLIA Kit, GRO CLIA Kit, Gro CLIA Kit, C-X-C motif chemokine ligand 1 CLIA Kit, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) CLIA Kit, C-X-C motif chemokine ligand 12 CLIA Kit, chemokine (C-X-C motif) ligand 1 CLIA Kit, CXCL1 CLIA Kit, Cxcl1 CLIA Kit, Cxcl12 CLIA Kit, GRO1 CLIA Kit

Hintergrund:

Gene Name: Neutrophil Activating Protein 3

Gene Aliases: CXCL1, GRO1, GROa, GRO-A, MGSA-A, NAP3, SCYB1, Chemokine C-X-C-Motif Ligand 1, Melanoma Growth Stimulating Activity Alpha, Fibroblast Secretory Protein

Gen-ID: 14825

UniProt: P12850

Pathways: Autophagie