Neuroserpin ELISA Kit

Produktnummer ABIN5657332

Produktdetails Neuroserpin ELISA Kit

Target: Neuroserpin (SERPINI1) (serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Neuroserpin (NSP). No significant cross-reactivity or interference between Neuroserpin (NSP) and analogues was observed.

Sensitivität: 0.34 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroserpin (NSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuroserpin (NSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuroserpin (NSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuroserpin (NSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuroserpin (NSP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuroserpin (NSP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Neuroserpin

Target: Neuroserpin (SERPINI1) (serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))

Andere Bezeichnung: Neuroserpin (SERPINI1 Produkte)

Synonyme: PI12 ELISA Kit, neuroserpin ELISA Kit, AI837402 ELISA Kit, Ns ELISA Kit, PI-12 ELISA Kit, Spi17 ELISA Kit, CG9453 ELISA Kit, Dmel\\CG9453 ELISA Kit, Serp2 ELISA Kit, Sp4 ELISA Kit, Spn4 ELISA Kit, Spn4A ELISA Kit, dSerp2 ELISA Kit, sp4 ELISA Kit, spn4 ELISA Kit, raPIT5a ELISA Kit, SERPINI1 ELISA Kit, pi12 ELISA Kit, serpini1l ELISA Kit, si:ch211-167c22.4 ELISA Kit, serpin family I member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade I, member 1 ELISA Kit, Serpin 42Da ELISA Kit, serpin peptidase inhibitor, clade I (neuroserpin), member 1 ELISA Kit, serpin family I member 1 L homeolog ELISA Kit, SERPINI1 ELISA Kit, Serpini1 ELISA Kit, Spn42Da ELISA Kit, serpini1 ELISA Kit, serpini1.L ELISA Kit

Hintergrund:

Gene Name: Neuroserpin

Gene Aliases: SERPINI1, PI12, Serpin Peptidase Inhibitor Clade I Member 1, Peptidase inhibitor 12

Gen-ID: 5274

UniProt: Q99574

Pathways: Regulation of Hormone Metabolic Process