Neuroserpin ELISA Kit
SERPINI1
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951558
-
-
Target
Alle Neuroserpin (SERPINI1) ELISA Kits anzeigen
-
Neuroserpin (SERPINI1)
(serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))
-
Reaktivität
-
Human
-
Nachweismethode
-
Colorimetric
-
Methodentyp
-
Sandwich ELISA
-
Applikation
-
ELISA
-
Verwendungszweck
-
Human SerpinI1 ELISA Kit.
-
Proben
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytische Methode
-
Quantitative
-
Produktmerkmale
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Bestandteile
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Benötigtes Material
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
Top Product
-
Discover our top product SERPINI1 ELISA Kit
-
-
-
Applikationshinweise
-
Optimal working dilution should be determined by the investigator.
-
Plattentyp
-
Pre-coated
-
Protokoll
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
Beschränkungen
-
Nur für Forschungszwecke einsetzbar
-
-
Haltbarkeit
-
6 months
-
-
Target
Alle Neuroserpin (SERPINI1) ELISA Kits anzeigen
-
Neuroserpin (SERPINI1)
(serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))
-
Andere Bezeichnung
-
SerpinI1 (SERPINI1 Produkte)
-
Synonyme
-
PI12 ELISA Kit, neuroserpin ELISA Kit, AI837402 ELISA Kit, Ns ELISA Kit, PI-12 ELISA Kit, Spi17 ELISA Kit, CG9453 ELISA Kit, Dmel\\CG9453 ELISA Kit, Serp2 ELISA Kit, Sp4 ELISA Kit, Spn4 ELISA Kit, Spn4A ELISA Kit, dSerp2 ELISA Kit, sp4 ELISA Kit, spn4 ELISA Kit, raPIT5a ELISA Kit, SERPINI1 ELISA Kit, pi12 ELISA Kit, serpini1l ELISA Kit, si:ch211-167c22.4 ELISA Kit, serpin family I member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade I, member 1 ELISA Kit, Serpin 42Da ELISA Kit, serpin peptidase inhibitor, clade I (neuroserpin), member 1 ELISA Kit, serpin family I member 1 L homeolog ELISA Kit, SERPINI1 ELISA Kit, Serpini1 ELISA Kit, Spn42Da ELISA Kit, serpini1 ELISA Kit, serpini1.L ELISA Kit
-
Gen-ID
-
5274
-
UniProt
-
Q99574
-
Pathways
-
Regulation of Hormone Metabolic Process
-