SERPING1 ELISA Kit

Produktnummer ABIN5653191

Produktdetails SERPING1 ELISA Kit

Target: SERPING1 (Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 ng/mL - 1000 ng/mL

Untere Nachweisgrenze: 15.62 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Complement 1 Inhibitor (C1INH). No significant cross-reactivity or interference between Complement 1 Inhibitor (C1INH) and analogues was observed.

Sensitivität: 5.9 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement 1 Inhibitor (C1INH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement 1 Inhibitor (C1INH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement 1 Inhibitor (C1INH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement 1 Inhibitor (C1INH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SERPING1

Target: SERPING1 (Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))

Andere Bezeichnung: Complement 1 Inhibitor (SERPING1 Produkte)

Synonyme: fb64f02 ELISA Kit, im:7159527 ELISA Kit, si:dkeyp-110g5.2 ELISA Kit, wu:fb60c09 ELISA Kit, wu:fb62a08 ELISA Kit, wu:fb64f02 ELISA Kit, LOC100136072 ELISA Kit, C1-INH ELISA Kit, C1INH ELISA Kit, C1nh ELISA Kit, C1IN ELISA Kit, C1NH ELISA Kit, HAE1 ELISA Kit, HAE2 ELISA Kit, serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 ELISA Kit, C1 inhibitor ELISA Kit, serpin family G member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade G, member 1 ELISA Kit, serping1 ELISA Kit, LOC100136072 ELISA Kit, SERPING1 ELISA Kit, Serping1 ELISA Kit

Hintergrund:

Gene Name: Complement 1 Inhibitor

Gene Aliases: SERPING1, C1IN, C1-INH, C1NH, HAE1, HAE2, Serpin Peptidase Inhibitor Clade G Member 1, Angioedema Hereditary, C1 Inhibitor, C1 Esterase Inhibitor

UniProt: P97290

Pathways: Komplementsystem