BMP2 CLIA Kit

Produktnummer ABIN5652261

Produktdetails BMP2 CLIA Kit

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Reaktivität: Ratte

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 2 (BMP2). No significant cross-reactivity or interference between Bone Morphogenetic Protein 2 (BMP2) and analogues was observed.

Sensitivität: 2.31 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 2 (BMP2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 2 (BMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bone Morphogenetic Protein 2 (BMP2) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Andere Bezeichnung: Bone Morphogenetic Protein 2 (BMP2 Produkte)

Synonyme: BDA2 CLIA Kit, BMP2A CLIA Kit, AI467020 CLIA Kit, Bmp2a CLIA Kit, BMP-2 CLIA Kit, xBMP-2 CLIA Kit, xbmp2 CLIA Kit, BMP2 CLIA Kit, bmp2a CLIA Kit, bmp2 CLIA Kit, wu:fc59d09 CLIA Kit, bone morphogenetic protein 2 CLIA Kit, bone morphogenetic protein 2 L homeolog CLIA Kit, Bone morphogenetic protein 2 CLIA Kit, bone morphogenetic protein 2a CLIA Kit, BMP2 CLIA Kit, Bmp2 CLIA Kit, bmp2.L CLIA Kit, bmp2 CLIA Kit, bmp2a CLIA Kit

Hintergrund:

Gene Name: Bone Morphogenetic Protein 2

Gene Aliases: BMP2A, BMP-2A, Hemochromatosis Modifier

Gen-ID: 29373

UniProt: P49001

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation