BMP2 ELISA Kit

Produktnummer ABIN411260

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human BMP2. Es wurde in 10 Publikationen zitiert.

Kurzübersicht für BMP2 ELISA Kit (ABIN411260)

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Bindungsspezifität: AA 283-396

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Osseous Tissue, Cell Culture Supernatant, Serum

Produktdetails BMP2 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: Sandwich Human BMP-2 ELISA Kit to quantitate Human BMP2 in bone tissue, cell culture supernatants and serum.

Analytische Methode: Quantitative

Spezifität: Expression system for standard: CHO
Immunogen sequence: Q283-R396

Capture antibody and Detection antibody: monoclonal antibody from mouse, monoclonal antibody from mouse

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 2 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 7.8%, 5.3%, 7%
Inter-Assay Precision (CV%): 7.9%, 6.1%, 7.9%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für BMP2 ELISA Kit (ABIN411260)

Jing, Hao, Xu, Liu, Xu, Luo, Meng: "Effects of local delivery of BMP2, zoledronate and their combination on bone microarchitecture, biomechanics and bone turnover in osteoporotic rabbits." in: Scientific reports, Vol. 6, pp. 28537, (2018) (PubMed).

Wang, You, Wei, Zu, Song, Li, Yan: "Modification of Human Umbilical Cord Blood Stem Cells Using Polyethylenimine Combined with Modified TAT Peptide to Enhance BMP-2 Production." in: BioMed research international, Vol. 2017, pp. 2971413, (2018) (PubMed).

Li, Zhou, Wang, Yang, Ding, Zhou: "Controlled dual delivery of BMP-2 and dexamethasone by nanoparticle-embedded electrospun nanofibers for the efficient repair of critical-sized rat calvarial defect." in: Biomaterials, Vol. 37, pp. 218-29, (2015) (PubMed).

Li, Yi, Jin, Duan, Min: "Effects of sequentially released BMP-2 and BMP-7 from PELA microcapsule-based scaffolds on the bone regeneration." in: American journal of translational research, Vol. 7, Issue 8, pp. 1417-28, (2015) (PubMed).

Han, Lin, Lu, Zhi, Wang, Meng, Jiang: "BMP2-encapsulated chitosan coatings on functionalized Ti surfaces and their performance in vitro and in vivo." in: Materials science & engineering. C, Materials for biological applications, Vol. 40, pp. 1-8, (2014) (PubMed).

Qiao, Zhang, Jin, Miao, Shi, Liu, Yuan, Liu, Li, Zheng, Zhang, Li, Yang, Sun: "Using poly(lactic-co-glycolic acid) microspheres to encapsulate plasmid of bone morphogenetic protein 2/polyethylenimine nanoparticles to promote bone formation in vitro and in vivo." in: International journal of nanomedicine, Vol. 8, pp. 2985-95, (2013) (PubMed).

Xu, Liu, Guo, Liu, Li: "Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas me?dicas e biolo?gicas / Sociedade Brasileira de Biofi?sica ... [et al.], Vol. 46, Issue 9, pp. 809-14, (2013) (PubMed).

Tian, Zhu, Qiu, Guan, Li, Zheng, Dong, Xiao: "Synthesis and characterization of UPPE-PLGA-rhBMP2 scaffolds for bone regeneration." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 32, Issue 4, pp. 563-70, (2012) (PubMed).

Ma, Ma, Guo, Zhang: "Expression of bone morphogenetic protein-2 and its receptors in epithelial ovarian cancer and their influence on the prognosis of ovarian cancer patients." in: Journal of experimental & clinical cancer research : CR, Vol. 29, pp. 85, (2010) (PubMed).

Akai, Wakisaka: "The neurohistochemistry of neuroregulators in the dental pulp." in: The Journal of Osaka University Dental School, Vol. 26, pp. 1-15, (1987) (PubMed).

Details zu BMP2

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Andere Bezeichnung: BMP2

Hintergrund:

Background: Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Gene Full Name: bone morphogenetic protein 2

Gen-ID: 650

UniProt: P12643

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation