MSH alpha ELISA Kit

Produktnummer ABIN5651656

Produktdetails

Target: MSH alpha

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 123.46 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 123.46 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Alpha-Melanocyte Stimulating Hormone (aMSH). No significant cross-reactivity or interference between Alpha-Melanocyte Stimulating Hormone (aMSH) and analogues was observed.

Sensitivität: 48.2 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Alpha-Melanocyte Stimulating Hormone (aMSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Alpha-Melanocyte Stimulating Hormone (aMSH) and unlabeled Alpha-Melanocyte Stimulating Hormone (aMSH) (Standards or samples) with the pre-coated antibody specific to Alpha-Melanocyte Stimulating Hormone (aMSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Alpha-Melanocyte Stimulating Hormone (aMSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Alpha-Melanocyte Stimulating Hormone (aMSH) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Melanocyte Stimulating Hormone (aMSH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Melanocyte Stimulating Hormone (aMSH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: MSH alpha

Andere Bezeichnung: Alpha-Melanocyte Stimulating Hormone (MSH alpha Produkte)

Synonyme: ACTH ELISA Kit, CLIP ELISA Kit, LPH ELISA Kit, MSH ELISA Kit, NPP ELISA Kit, POC ELISA Kit, AMSH ELISA Kit, BE ELISA Kit, Beta-LPH ELISA Kit, Clip ELISA Kit, Gamma-LPH ELISA Kit, Npp ELISA Kit, Pomc-1 ELISA Kit, Pomc1 ELISA Kit, alpha-MSH ELISA Kit, alphaMSH ELISA Kit, beta-MSH ELISA Kit, gamma-MSH ELISA Kit, Pomc2 ELISA Kit, proopiomelanocortin ELISA Kit, STAM binding protein ELISA Kit, pro-opiomelanocortin-alpha ELISA Kit, POMC ELISA Kit, STAMBP ELISA Kit, Pomc ELISA Kit

Hintergrund:

Gene Name: Alpha-Melanocyte Stimulating Hormone

Gene Aliases: α-MSH, Intermedins, Alpha-Melanotropin, Alpha-Melanocortin, Alpha-Intermedin

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity