ADIPOQ ELISA Kit

Produktnummer ABIN5068043

Produktdetails ADIPOQ ELISA Kit

Target: ADIPOQ (Adiponectin (ADIPOQ))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: This ELISA is a sandwich enzyme immunoassay for the quantitative measurement of mouse Adiponectin/Acrp30 protein in serum. It is intended for in vitro research use only.

Proben: Serum

Analytische Methode: Quantitative

Sensitivität: 0.1 ng/mL

Produktmerkmale: Adiponectin, Mouse ELISA Kit

Anwendungsinformationen

Protokoll: Principle:

• The surface of the wells in a microtitration plate is coated with monoclonal anti-mouse Adiponectin specific antibody. The standards, quality controls (QC), and diluted samples are pipetted into the wells. Any mouse Adiponectin/Acrp30 present is captured by immobilized antibody and unbound protein is washed away after the first incubation period. Then, a horseradish peroxidase (HRP) conjugated polyclonal anti-mouse Adiponectin antibody is added to the wells and incubated. Following another washing step, to remove unbound antibody-HRP conjugate, a substrate solution (H2O2 and TMB) is added to the wells. The enzymatic reaction yields a blue product that turns yellow when acidic stop solution is added. The intensity of the color, measured spectrophotochemically at 450nm, is directly proportional to the amount of mouse Adiponectin bound in the initial step. The concentrations of the diluted test samples are then read off the standard curve that is constructed by plotting the absorbance values against each respective mouse Adiponectin standard level using a four-parameter function. The dilution factor needs to be taken into consideration when calculating the actual concentration of Adiponectin/Acrp30 analyte in the test samples.

Testpräzision: Precision pipettes to deliver 10-1000ul and disposable tips

• 3. Multichannel pipette 50-200ul
• 4. Microplate reader with 450 ± 10nm filter
• 5. Orbital microplate shaker capable of agitation at approximately 300rpm
• 6. Software package facilitating data generation and analysis
• 7. Microplate washer (optional). Manual washing is possible but not preferable.
• 8. Glassware (graduated cylinder and bottle for Wash Solution)
• 9. Distilled water

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: 4°C/-70°C

Details zu ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Andere Bezeichnung: Adiponectin (ADIPOQ Produkte)

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