Adiponectin (ADIPOQ) ELISA Kit

Details zu Produkt Nr. ABIN411385, Anbieter: Anmelden zum Anzeigen
Antigen
  • ACDC
  • ACRP30
  • ADIPQTL1
  • ADPN
  • APM-1
  • APM1
  • GBP28
  • apM-1
  • 30kDa
  • APN
  • Acdc
  • Acrp30
  • Ad
  • adipo
  • apM1
  • ADN
  • adipoq
  • adiponectin, C1Q and collagen domain containing
  • adiponectin, C1Q and collagen domain containing L homeolog
  • ADIPOQ
  • Adipoq
  • adipoq.L
Epitop
AA 18-247
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
55
40
32
12
9
8
8
7
6
3
2
2
1
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
312-20000 pg/mL
Untere Nachweisgrenze
312 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Adiponectin
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate, Urine
Analytische Methode Quantitative
Nachweismethode Colorimetric
Immunogen Expression system for standard: NSO
Immunogen sequence: E18-N247
Spezifität Expression system for standard: NSO
Immunogen sequence: E18-N247
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Andere Bezeichnung ADIPOQ (ADIPOQ ELISA Kit Abstract)
Hintergrund

Protein Function: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. .

Background: Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.

Synonyms: Adiponectin,30 kDa adipocyte complement-related protein,Adipocyte complement-related 30 kDa protein,ACRP30,Adipocyte, C1q and collagen domain-containing protein,Adipocyte-specific protein AdipoQ,Adipoq,Acdc, Acrp30, Apm1,

Full Gene Name: Adiponectin

Cellular Localisation: Secreted.
Gen-ID 11450
UniProt Q60994
Applikations-hinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Sequence similarities: Contains 1 C1q domain.

Tissue Specificity: Synthesized exclusively by adipocytes and secreted into plasma.

Plattentyp Pre-coated
Protokoll mouse Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Adiponectin has been precoated onto 96-well plates. Standards(NSO, E18-N247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Adiponectin amount of sample captured in plate.
Testdurchführung

Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse Adiponectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Adiponectin standard solution and each sample be measured in duplicate.

Testpräzision
  • Sample 1: n=16, Mean(ng/ml): 3.35, Standard deviation: 0.160, CV(%): 5
  • Sample 2: n=16, Mean(ng/ml): 6.82, Standard deviation: 0.360, CV(%): 5.3
  • Sample 3: n=16, Mean(ng/ml): 13.50, Standard deviation: 0.756, CV(%): 5.6,
  • Sample 1: n=24, Mean(ng/ml): 3.39, Standard deviation: 0.190, CV(%): 5.4
  • Sample 2: n=24, Mean(ng/ml): 6.60, Standard deviation: 0.380, CV(%): 5.8
  • Sample 3: n=24, Mean(ng/ml): 13.20, Standard deviation: 0.818, CV(%): 6.2
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months
Bilder des Herstellers
ELISA image for Adiponectin (ADIPOQ) ELISA Kit (ABIN411385) Mouse Adiponectin PicoKine ELISA Kit standard curve
Produkt verwendet in: Chen, Wang, Hu, Zhou, Xu, Qiu, Qin, Zhang, Ying: "Programming of mouse obesity by maternal exposure to concentrated ambient fine particles." in: Particle and fibre toxicology, Vol. 14, Issue 1, pp. 20, 2017 (PubMed).

Rasekhi, Karandish, Jalali, Mohammadshahi, Zarei, Saki, Shahbazian: "Phylloquinone supplementation improves glycemic status independent of the effects of adiponectin levels in premonopause women with prediabetes: a double-blind randomized controlled clinical trial." in: Journal of diabetes and metabolic disorders, Vol. 14, Issue 1, pp. 1, 2015 (PubMed).

Wu, Weng, Lai, Tsai, Liu, Lee, Tsai: "Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 391767, 2015 (PubMed).

Qin, Ma, Yang, Hu, Zhou, Fu, Tian, Liu, Xu, Shen: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." in: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, 2015 (PubMed).

Liu, Gu, Zhao, An, Yang: "Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor ? agonist rosiglitazone treatment." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1813-1818, 2014 (PubMed).

Liu, Liu, Li, Han, Peng, Shen: "Adiponectin is expressed in the pancreas of high-fat-diet-fed mice and protects pancreatic endothelial function during the development of type 2 diabetes." in: Diabetes & metabolism, Vol. 40, Issue 5, pp. 363-72, 2014 (PubMed).

Hatami Zargaran, Salehi, Heydari, Babajafari: "The Effects of 6 Isocaloric Meals on Body Weight, Lipid Profiles, Leptin, and Adiponectin in Overweight Subjects (BMI > 25)." in: International cardiovascular research journal, Vol. 8, Issue 2, pp. 52-6, 2014 (PubMed).

Chen, Li, Zhang, Li, Sun, Zhang, Feng, Zhu: "Effects of resveratrol on the amelioration of insulin resistance in KKAy mice." in: Canadian journal of physiology and pharmacology, Vol. 90, Issue 2, pp. 237-42, 2012 (PubMed).

Allgemeine Veröffentlichungen Hu, Shu, Guo, Ma: "Effect of an Ilex asprella root decoction on the related genes of lipid metabolism from chronic stress and hyperlipidemic fatty liver in rats." in: Chinese medical journal, Vol. 125, Issue 19, pp. 3539-42, 2012 (PubMed).

Yamauchi, Kamon, Minokoshi, Ito, Waki, Uchida, Yamashita, Noda, Kita, Ueki, Eto, Akanuma, Froguel, Foufelle, Ferre, Carling, Kimura, Nagai, Kahn, Kadowaki: "Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase." in: Nature medicine, Vol. 8, Issue 11, pp. 1288-95, 2002 (PubMed).

Yokota, Oritani, Takahashi, Ishikawa, Matsuyama, Ouchi, Kihara, Funahashi, Tenner, Tomiyama, Matsuzawa: "Adiponectin, a new member of the family of soluble defense collagens, negatively regulates the growth of myelomonocytic progenitors and the functions of macrophages." in: Blood, Vol. 96, Issue 5, pp. 1723-32, 2000 (PubMed).

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