HMGB1 ELISA Kit

Produktnummer ABIN5067936

Produktdetails HMGB1 ELISA Kit

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: The Hmgb1 (High mobility group protein B1) ELISA Kit is a quantitative sandwich assay for the detection of Hmgb1 in mouse serum, plasma, tissue homogenates and other biological fluids.

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 9.375 pg/mL

Produktmerkmale: Hmgb1 (High mobility group protein B1) ELISA Kit (Mouse)

Bestandteile:

• *356299A: Microtiter Strips, 1x96 wells (8x12 wells).
• *356299B: Standard, 2x1 vial
• 356299C: Sample/Standard Dilution Buffer, 1x20ml
• 356299D: Antibody (Biotin) (Concentrated), 1x120ul
• 356299E: Antibody Dilution Buffer, 1x10ml
• 356299F: Streptavidin (HRP) (SABC), 1x120ul
• 356299G: SABC Dilution Buffer, 1x10ml
• 356299H: TMB Substrate, 1x10ml
• 356299J: Stop Solution, 1x10ml
• 356299K: Wash Buffer, 25X, 1x30ml

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Principle:

• This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate provided in this kit has been pre-coated with an antibody specific to Hmgb1. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hmgb1, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hmgb1 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Testpräzision: Precision:

• Intra-assay CV: <8%
• Inter-assay CV: <10%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C/-20°C

Details zu HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: Hmgb1 (High mobility group protein B1) (HMGB1 Produkte)

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Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome