EPO ELISA Kit

Produktnummer ABIN5026954

Produktdetails EPO ELISA Kit

Target: EPO (Erythropoietin (EPO))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25-2000 pg/mL

Untere Nachweisgrenze: 31.25 pg/mL

Applikation: ELISA

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of Human EPO concentrations in serum, plasma and other biological fluids.

Proben: Plasma, Serum

Analytische Methode: Quantitative

Sensitivität: < 18.75 pg/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-human EPO coated on a 96-well plate. Standards and samples are pipetted into the wells and EPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human EPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Details zu EPO

Target: EPO (Erythropoietin (EPO))

Andere Bezeichnung: EPO (Erythropoietin) (EPO Produkte)

Synonyme: EPO ELISA Kit, EP ELISA Kit, MVCD2 ELISA Kit, erythropoietin ELISA Kit, erythropoietin S homeolog ELISA Kit, erythropoietin a ELISA Kit, EPO ELISA Kit, epo ELISA Kit, epo.S ELISA Kit, Epo ELISA Kit, epoa ELISA Kit

Substanzklasse: Hormone

Pathways: JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity