EPO ELISA Kit (Erythropoietin)

Details for Product EPO ELISA Kit No. ABIN5026954, Anbieter: Anmelden zum Anzeigen
Antigen
  • EPO
  • EP
  • MVCD2
  • erythropoietin
  • erythropoietin S homeolog
  • erythropoietin a
  • EPO
  • epo
  • epo.S
  • Epo
  • epoa
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
25
24
24
15
13
9
6
6
5
4
4
3
3
3
2
Methodentyp
Sandwich ELISA
Detektionsbereich
31.25-2000 pg/mL
Untere Nachweisgrenze
31.25 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Verwendungszweck This immunoassay kit allows for the in vitro quantitative determination of Human EPO concentrations in serum, plasma and other biological fluids.
Proben Plasma, Serum
Analytische Methode Quantitative
Nachweismethode Colorimetric
Sensitivität < 18.75 pg/mL
Bestandteile plate, standard, diluent, antibodies
Benötigtes Material pipettes, tubes, reader, incubator
Plasmids, Primers & others Plasmide, Primers & weitere EPO products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung EPO (Erythropoietin) (EPO ELISA Kit Abstract)
Substanzklasse Hormone
Pathways JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
Probenmenge 100 μL
Testdauer 3 - 4 h
Plattentyp Pre-coated
Protokoll This assay employs an antibody specific for Anti-human EPO coated on a 96-well plate. Standards and samples are pipetted into the wells and EPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human EPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Vorsichtsmaßnahmen protect your eyes
Lagerung 4 °C
Bilder des Herstellers
ELISA image for Erythropoietin (EPO) ELISA Kit (ABIN5026954) Erythropoietin (EPO) ELISA Kit
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