PLA2G7 ELISA Kit

Produktnummer ABIN4889779

Produktdetails PLA2G7 ELISA Kit

Target: PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 8.19-2.000 ng/mL

Untere Nachweisgrenze: 8.19 ng/mL

Applikation: ELISA

Verwendungszweck: Human Lp-PLA2/PLA2G7/PAF-AH ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human Lp-PLA2.

Sensitivität: 8.1 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Reagenzien:

Recommended Dilution for serum and plasma samples2 fold

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu PLA2G7

Target: PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))

Andere Bezeichnung: Lp-PLA2 (PLA2G7/PAF-AH) (Lp-PLA2 Produkte)

Synonyme: LDL-PLA2 ELISA Kit, LP-PLA2 ELISA Kit, PAFAD ELISA Kit, PAFAH ELISA Kit, Lp-PLA2 ELISA Kit, PAF-AH ELISA Kit, R75400 ELISA Kit, zgc:77563 ELISA Kit, phospholipase A2 group VII ELISA Kit, phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) ELISA Kit, PLA2G7 ELISA Kit, Pla2g7 ELISA Kit, pla2g7 ELISA Kit

Gen-ID: 7941

UniProt: Q13093

Pathways: Peptide Hormone Metabolism