EPO ELISA Kit (Erythropoietin)

Details for Product EPO ELISA Kit No. ABIN4885513, Anbieter: Anmelden zum Anzeigen
Antigen
  • EPO
  • EP
  • MVCD2
  • erythropoietin
  • erythropoietin S homeolog
  • erythropoietin a
  • EPO
  • epo
  • epo.S
  • Epo
  • epoa
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
25
24
24
15
13
9
6
6
5
4
4
3
3
3
2
1
Methodentyp
Sandwich ELISA
Detektionsbereich
1.5-400 pg/mL
Untere Nachweisgrenze
1.5 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Verwendungszweck Mouse Erythropoietin ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Proben Plasma, Cell Culture Supernatant, Serum
Analytische Methode Quantitative
Nachweismethode Colorimetric
Spezifität The antibody pair provided in this kit recognizes Mouse Erythropoietin (EPO)
Produktmerkmale
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Bestandteile
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Benötigtes Material
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmide, Primers & weitere EPO products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung Erythropoietin (EPO ELISA Kit Abstract)
Substanzklasse Hormone
Gen-ID 13856
UniProt P07321
Pathways JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
Applikations-hinweise Recommended Dilution for serum and plasma samples2 fold
Probenmenge 100 μL
Plattentyp Pre-coated
Protokoll
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung -20 °C
Informationen zur Lagerung The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit 6 months
Bilder des Herstellers
ELISA image for Erythropoietin (EPO) ELISA Kit (ABIN4885513) Erythropoietin (EPO) ELISA Kit
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