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WNT4 ELISA Kit
WNT4
Reaktivität: Human
Colorimetric
Sandwich ELISA
1.22-300 ng/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4885058
Produktdetails WNT4 ELISA Kit
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Target
Alle WNT4 ELISA Kits anzeigen
WNT4
(Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))
Reaktivität
Alle Reaktivitäten für WNT4 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
1.22-300 ng/mL
Untere Nachweisgrenze
1.22 ng/mL
Applikation
ELISA
Verwendungszweck
Human Wnt-4 ELISA Kit for serum, plasma, and cell culture supernatant samples.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair detects human Wnt-4. Other species not determined.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples2 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu WNT4
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Target
Alle WNT4 ELISA Kits anzeigen
WNT4
(Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))
Andere Bezeichnung
Wnt-4 (WNT4 Produkte )
Synonyme
CG4698 ELISA Kit, DWnt-4 ELISA Kit, DWnt4 ELISA Kit, Dm DWnt4 ELISA Kit, Dmel\\CG4698 ELISA Kit, Dwnt4 ELISA Kit, Wnt ELISA Kit, Wnt-4 ELISA Kit, anon-EST:Liang-2.4 ELISA Kit, clone 2.4 ELISA Kit, wnt-4 ELISA Kit, wnt4 ELISA Kit, SERKAL ELISA Kit, WNT-4 ELISA Kit, Xwnt4 ELISA Kit, xwnt-4 ELISA Kit, zgc:136737 ELISA Kit, Wnt family member 4 ELISA Kit, Wnt oncogene analog 4 ELISA Kit, wingless-type MMTV integration site family, member 4 ELISA Kit, wingless-type MMTV integration site family member 4 S homeolog ELISA Kit, wingless-type MMTV integration site family, member 4a ELISA Kit, WNT4 ELISA Kit, Wnt4 ELISA Kit, wnt4.S ELISA Kit, wnt4a ELISA Kit
Gen-ID
54361
UniProt
P56705
Pathways
WNT Signalweg , Regulation of Hormone Metabolic Process , Regulation of Hormone Biosynthetic Process , Cell-Cell Junction Organization , Tube Formation
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