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NTRK3 ELISA Kit
NTRK3
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4884904
Produktdetails NTRK3 ELISA Kit
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Target
Alle NTRK3 ELISA Kits anzeigen
NTRK3
(Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3))
Reaktivität
Alle Reaktivitäten für NTRK3 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human TRKC ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes human TRKC. Other species not yet determined.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu NTRK3
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Target
Alle NTRK3 ELISA Kits anzeigen
NTRK3
(Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3))
Andere Bezeichnung
TRKC (NTRK3 Produkte )
Synonyme
TRKC ELISA Kit, gp145(trkC) ELISA Kit, trkC ELISA Kit, AW125844 ELISA Kit, Ntrk3_tv3 ELISA Kit, TrkC ELISA Kit, neurotrophic receptor tyrosine kinase 3 ELISA Kit, neurotrophic tyrosine kinase, receptor, type 3 ELISA Kit, NTRK3 ELISA Kit, Ntrk3 ELISA Kit
Gen-ID
4916
UniProt
Q16288
Pathways
RTK Signalweg , Neurotrophin Signalübertragung , Regulation of Cell Size
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