×
Aktivieren Sie Javascript in Ihrem Browser, um unsere Webseite optimal zu nutzen.
SPI1 ELISA Kit
SPI1
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4884304
Produktdetails SPI1 ELISA Kit
(ausblenden)
Target
Alle SPI1 ELISA Kits anzeigen
SPI1
(Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
Reaktivität
Alle Reaktivitäten für SPI1 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human PU.1/Spi-1 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes .
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Top Product
Discover our top product SPI1 ELISA Kit
Alternativen
(anzeigen)
Anwendungsinformationen
(ausblenden)
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
(ausblenden)
Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu SPI1
(ausblenden)
Target
Alle SPI1 ELISA Kits anzeigen
SPI1
(Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
Andere Bezeichnung
PU.1 (SPI1 Produkte )
Synonyme
OF ELISA Kit, PU.1 ELISA Kit, SFPI1 ELISA Kit, SPI-1 ELISA Kit, SPI-A ELISA Kit, Dis-1 ELISA Kit, Dis1 ELISA Kit, Sfpi-1 ELISA Kit, Sfpi1 ELISA Kit, Spi-1 ELISA Kit, Tcfpu1 ELISA Kit, Tfpu.1 ELISA Kit, Pu.1 ELISA Kit, cb1086 ELISA Kit, pu.1 ELISA Kit, pu1 ELISA Kit, sfpi1 ELISA Kit, si:by184l24.2 ELISA Kit, spi1 ELISA Kit, SPI1/PU.1 ELISA Kit, spi1l ELISA Kit, zgc:152991 ELISA Kit, Spi-1 proto-oncogene ELISA Kit, spleen focus forming virus (SFFV) proviral integration oncogene ELISA Kit, Spi-1 proto-oncogene b ELISA Kit, Spi-1 proto-oncogene a ELISA Kit, SPI1 ELISA Kit, Spi1 ELISA Kit, spi1b ELISA Kit, spi1a ELISA Kit
Gen-ID
6688
Pathways
Stem Cell Maintenance
Zuletzt angesehen
(ausblenden)