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SPI1 ELISA Kit

SPI1 Reaktivität: Human Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5674925
  • Target Alle SPI1 ELISA Kits anzeigen
    SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
    Reaktivität
    • 4
    • 3
    • 3
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    78.125 pg/mL - 5000 pg/mL
    Untere Nachweisgrenze
    78.125 pg/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Sensitivität
    33.2 pg/mL
    Bestandteile
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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    Discover our top product SPI1 ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle SPI1 ELISA Kits anzeigen
    SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
    Andere Bezeichnung
    Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) (SPI1 Produkte)
    Synonyme
    OF ELISA Kit, PU.1 ELISA Kit, SFPI1 ELISA Kit, SPI-1 ELISA Kit, SPI-A ELISA Kit, Dis-1 ELISA Kit, Dis1 ELISA Kit, Sfpi-1 ELISA Kit, Sfpi1 ELISA Kit, Spi-1 ELISA Kit, Tcfpu1 ELISA Kit, Tfpu.1 ELISA Kit, Pu.1 ELISA Kit, cb1086 ELISA Kit, pu.1 ELISA Kit, pu1 ELISA Kit, sfpi1 ELISA Kit, si:by184l24.2 ELISA Kit, spi1 ELISA Kit, SPI1/PU.1 ELISA Kit, spi1l ELISA Kit, zgc:152991 ELISA Kit, Spi-1 proto-oncogene ELISA Kit, spleen focus forming virus (SFFV) proviral integration oncogene ELISA Kit, Spi-1 proto-oncogene b ELISA Kit, Spi-1 proto-oncogene a ELISA Kit, SPI1 ELISA Kit, Spi1 ELISA Kit, spi1b ELISA Kit, spi1a ELISA Kit
    Hintergrund
    Alternative name: OF, PU.1, SFPI1, SPI-1, SPI-A, SPI-1 Proto-Oncogene, Hematopoietic Transcription Factor PU.1, 31 kDa Transforming Protein
    Gen-ID
    6688
    UniProt
    P17947
    Pathways
    Stem Cell Maintenance
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