Cytokeratin 18 ELISA Kit

Produktnummer ABIN4882548

Produktdetails Cytokeratin 18 ELISA Kit

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.164-40 ng/mL

Untere Nachweisgrenze: 0.164 ng/mL

Applikation: ELISA

Verwendungszweck: Human Cytokeratin 18/KRT18 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human Cytokeratin 18.

Sensitivität: 160 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Andere Bezeichnung: Cytokeratin 18 (KRT18 Produkte)

Synonyme: CK18 ELISA Kit, K18 ELISA Kit, Krt1-18 ELISA Kit, CYK18 ELISA Kit, DreK18 ELISA Kit, cb83 ELISA Kit, dapk1 ELISA Kit, sb:cb83 ELISA Kit, wu:fa13f02 ELISA Kit, wu:fb36b04 ELISA Kit, krt18 ELISA Kit, MGC64569 ELISA Kit, MGC75922 ELISA Kit, KRT18 ELISA Kit, CK-18-A ELISA Kit, K18-A ELISA Kit, NC-11 ELISA Kit, cyk18 ELISA Kit, k18 ELISA Kit, krt18-a ELISA Kit, krt18-b ELISA Kit, krt18a ELISA Kit, xkendob ELISA Kit, keratin 18 ELISA Kit, keratin 18, type I L homeolog ELISA Kit, keratin 18, type I ELISA Kit, keratin, type I cytoskeletal 18 ELISA Kit, K18, simple type I keratin ELISA Kit, Keratin, type I cytoskeletal 18 ELISA Kit, keratin 18, type I S homeolog ELISA Kit, Krt18 ELISA Kit, KRT18 ELISA Kit, krt18 ELISA Kit, krt18.L ELISA Kit, LOC700569 ELISA Kit, LOC100136778 ELISA Kit, k1c18 ELISA Kit, LOC100008885 ELISA Kit, krt18.S ELISA Kit

Gen-ID: 3875

UniProt: P05783

Pathways: Apoptose, Caspase Kaskade in der Apoptose