Caspase 3 ELISA Kit
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- Target Alle Caspase 3 (CASP3) ELISA Kits anzeigen
- Caspase 3 (CASP3)
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Applikation
- ELISA
- Verwendungszweck
- Custom Human Caspase-3 ELISA Kit.
- Proben
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytische Methode
- Quantitative
- Spezifität
- This ELISA antibody pair recognizes Human Fes.
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Top Product
- Discover our top product CASP3 ELISA Kit
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- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle Caspase 3 (CASP3) ELISA Kits anzeigen
- Caspase 3 (CASP3)
- Andere Bezeichnung
- Caspase-3 (CASP3 Produkte)
- Synonyme
- CPP32 ELISA Kit, CPP32B ELISA Kit, SCA-1 ELISA Kit, A830040C14Rik ELISA Kit, AC-3 ELISA Kit, Apopain ELISA Kit, CC3 ELISA Kit, Caspase-3 ELISA Kit, Lice ELISA Kit, Yama ELISA Kit, mldy ELISA Kit, xcpp32 ELISA Kit, casp3 ELISA Kit, zgc:100890 ELISA Kit, CASP-3 ELISA Kit, caspase-3 ELISA Kit, caspase 3 ELISA Kit, caspase 3 S homeolog ELISA Kit, caspase 3, apoptosis-related cysteine peptidase a ELISA Kit, caspase 3, apoptosis-related cysteine peptidase ELISA Kit, CASP3 ELISA Kit, Casp3 ELISA Kit, casp3.S ELISA Kit, casp3a ELISA Kit
- Gen-ID
- 836
- UniProt
- P42574
- Pathways
- Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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