AKR1C3 ELISA Kit

Produktnummer ABIN4881786

Produktdetails AKR1C3 ELISA Kit

Target: AKR1C3 (Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human AKR1C3 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human BIRC4.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu AKR1C3

Target: AKR1C3 (Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))

Andere Bezeichnung: AKR1C3 (AKR1C3 Produkte)

Synonyme: AKR1C3 ELISA Kit, PGFS ELISA Kit, MGC134333 ELISA Kit, DD3 ELISA Kit, DDX ELISA Kit, HA1753 ELISA Kit, HAKRB ELISA Kit, HAKRe ELISA Kit, HSD17B5 ELISA Kit, hluPGFS ELISA Kit, Akr1c18 ELISA Kit, aldo-keto reductase family 1, member C3 L homeolog ELISA Kit, aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II) ELISA Kit, aldo-keto reductase family 1 member C2 ELISA Kit, aldo-keto reductase family 1 member C3 ELISA Kit, aldo-keto reductase family 1, member C3 ELISA Kit, akr1c3.L ELISA Kit, AKR1C3 ELISA Kit, LOC722496 ELISA Kit, Akr1c3 ELISA Kit

Gen-ID: 8644

UniProt: P42330

Pathways: Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Biosynthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, C21-Steroid Hormone Metabolic Process, Protein targeting to Nucleus