AGRP FLISA Kit
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- Target Alle AGRP FLISA Kits anzeigen
- AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))
- Bindungsspezifität
- AA 71-132, amidated
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Reaktivität
- Human
- Nachweismethode
- Fluorometric
- Methodentyp
- Competition ELISA
- Applikation
- ELISA
- Analytische Methode
- Quantitative
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Plattentyp
- Pre-coated
- Protokoll
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C
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- Target Alle AGRP FLISA Kits anzeigen
- AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))
- Andere Bezeichnung
- Agouti-Related Protein (AGRP Produkte)
- Synonyme
- AGRT FLISA Kit, ART FLISA Kit, ASIP2 FLISA Kit, Agrt FLISA Kit, Art FLISA Kit, agouti related neuropeptide FLISA Kit, agouti-related protein pseudogene FLISA Kit, AGRP FLISA Kit, Agrp FLISA Kit, LOC100352591 FLISA Kit
- Pathways
- Feeding Behaviour, Photoperiodism
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