AGRP ELISA Kit

Produktnummer ABIN3044701

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human AGRP.

Kurzübersicht für AGRP ELISA Kit (ABIN3044701)

Target: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Bindungsspezifität: AA 21-132

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8 pg/mL - 500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Urine, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Produktdetails AGRP ELISA Kit

Untere Nachweisgrenze: 7.8 pg/mL

Verwendungszweck: Sandwich Human AGRP/agouti-related ELISA Kit to quantitate Human AGRP in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: A21-T132

Capture antibody and Detection antibody: a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 4 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 4.2%, 4%, 4.4%
Inter-Assay Precision (CV%): 5.3%, 5.3%, 4.9%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Details zu AGRP

Target: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Andere Bezeichnung: AGRP

Hintergrund:

Background: Agouti-related protein, also called Agouti-related peptide (AgRP), is a neuropeptide produced in the brain by the AgRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. In humans, the agouti-related peptide is encoded by the AGRP gene. AgRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators.

Gene Full Name: agouti related neuropeptide

Gen-ID: 181

UniProt: O00253

Pathways: Feeding Behaviour, Photoperiodism