FGF2 ELISA Kit

Produktnummer ABIN2747985

Produktdetails FGF2 ELISA Kit

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 2-500 pg/mL

Untere Nachweisgrenze: 2 pg/mL

Applikation: ELISA

Verwendungszweck: Rat bFGF ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: The antibody pair provided in this kit recognizes Rat bFGF (FGF2)

Sensitivität: 2 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Referenzen für FGF2 ELISA Kit (ABIN2747985)

Zhang, Zhang, Liu, Zhang, Zhang, Tang: "Repairing sciatic nerve injury with an EPO-loaded nerve conduit and sandwiched-in strategy of transplanting mesenchymal stem cells." in: Biomaterials, Vol. 142, pp. 90-100, (2018) (PubMed).

Details zu FGF2

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Andere Bezeichnung: bFGF (FGF2 Produkte)

Synonyme: BFGF ELISA Kit, FGF-2 ELISA Kit, FGFB ELISA Kit, HBGF-2 ELISA Kit, Fgf-2 ELISA Kit, Fgfb ELISA Kit, bFGF ELISA Kit, fibroblast growth factor 2 ELISA Kit, fibroblast growth factor 2 (basic) ELISA Kit, FGF2 ELISA Kit, Fgf2 ELISA Kit, fgf2 ELISA Kit

Hintergrund: Gene Names: Fgf2 Fgf-2
Protein names: Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)

Gen-ID: 54250

UniProt: P13109

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteine