ADIPOQ ELISA Kit
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- Target Alle ADIPOQ ELISA Kits anzeigen
- ADIPOQ (Adiponectin (ADIPOQ))
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Reaktivität
- Human, Rhesusaffen
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 40.96-10.000 ng/mL
- Untere Nachweisgrenze
- 40.96 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- Rhesus Macaque Adiponectin/ACRP30 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- Proben
- Plasma, Cell Culture Supernatant, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This ELISA antibody pair recognizes Rhesus Macaque Adiponectin.
- Sensitivität
- 40 pg/mL
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Applikationshinweise
- Recommended Dilution for serum and plasma samples20,000 fold
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Testdurchführung
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle ADIPOQ ELISA Kits anzeigen
- ADIPOQ (Adiponectin (ADIPOQ))
- Andere Bezeichnung
- Adiponectin (ADIPOQ Produkte)
- Synonyme
- ADP ELISA Kit, DCAF9 ELISA Kit, 6720489L24Rik ELISA Kit, 9330209L04Rik ELISA Kit, Adp ELISA Kit, Mamdc1 ELISA Kit, RNSVSP4U ELISA Kit, SVSIV1 ELISA Kit, Svp4 ELISA Kit, ACDC ELISA Kit, ACRP30 ELISA Kit, ADIPQTL1 ELISA Kit, ADPN ELISA Kit, APM-1 ELISA Kit, APM1 ELISA Kit, GBP28 ELISA Kit, apM-1 ELISA Kit, 30kDa ELISA Kit, APN ELISA Kit, Acdc ELISA Kit, Acrp30 ELISA Kit, Ad ELISA Kit, adipo ELISA Kit, apM1 ELISA Kit, ADN ELISA Kit, acrpl ELISA Kit, adipo-b ELISA Kit, adipoql ELISA Kit, zgc:153584 ELISA Kit, adipoq ELISA Kit, WD and tetratricopeptide repeats 1 ELISA Kit, MAM domain containing glycosylphosphatidylinositol anchor 2 ELISA Kit, seminal vesicle secretory protein 4 ELISA Kit, adiponectin, C1Q and collagen domain containing ELISA Kit, adiponectin, C1Q and collagen domain containing, b ELISA Kit, adiponectin, C1Q and collagen domain containing L homeolog ELISA Kit, WDTC1 ELISA Kit, Mdga2 ELISA Kit, Svs4 ELISA Kit, ADIPOQ ELISA Kit, Adipoq ELISA Kit, adipoqb ELISA Kit, adipoq.L ELISA Kit
- Hintergrund
- Adiponectin (ACRP30)
- Gen-ID
- 574212
- UniProt
- F7HQ03
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